Comparison of immunomodulatory effects of placenta mesenchymal stem cells with bone marrow and adipose mesenchymal stem cells

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• 作者：Jung Min Lee^a ; Jieun Jung^b ; Hyun-Jung Lee^b ; Su Jin Jeong^c ; Kyung Jin Cho^d ; Seong-Gyu Hwang^a ; Gi Jin Kim^b ; ^{gjkim@cha.ac.kr}
• 关键词：AD-MSCs ; adipose-derived MSCs ; AFP ; a-fetoprotein ; BM-MSCs ; bone marrow-derived MSCs ; CCl4 ; carbon tetrachloride ; CP-MSCs ; chorionic plate-derived mesenchymal stem cells ; GM-CSF ; granulocyte macrophage colony-stimulating factor ; HLA ; human leukocyte antig
• 刊名：International Immunopharmacology
• 出版年：2012
• 期刊代码：123_15675769
• 类别：med
• 出版时间：June, 2012
• 卷：13
• 期：2
• 页码：219-224
• 文件大小：754 K

摘要

Mesenchymal stem cells (MSCs) are powerful sources for cell therapy in regenerative medicine because they can be isolated from various tissues, expanded, and induced into multiple-lineages. Of note, their immunomodulatory effects maximize the therapeutic effects of stem cells engrafted on host, making them an especially attractive choice. Recently, several varieties of placenta-derived stem cells (PDSCs) including chorionic plate-derived MSCs (CP-MSCs) have been suggested as alternative sources of stem cells. However, comparative studies of immunomodulatory effects for CP-MSCs among various MSCs are largely lacking. We examined and compared immunomodulatory function of CP-MSCs with that of BM-MSCs and AD-MSCs using co-culture system with activated T-cells derived from human umbilical cord blood (UCB) exposed to anti-CD3 and anti-CD28 which are T-cell activating monoclonal antibodies. All MSCs expressed markers of stem cells and three germ layers by RT-PCR. These cells also exhibited comparable immunomodulatory effects when they were co-cultured with activated T-cells in dose-dependent manner. However, expression of HLA-ABC and HLA-G was highly positive in CP-MSCs compared to other MSCs, and higher levels of cytokines of IL-2, IL-4, IL-13, and GM-CSF were detected in dose-dependent manner in CP-MSCs. Taken together, the results of the present study suggest that while CP-MSCs, BM-MSCs, and AD-MSCs all have immunomodulatory effects, CP-MSCs may have additional advantage over the other MSCs in terms of immunomodulation. In conjunction with other previous studies, CP-MSCs are suggested to be a useful stem cell source in cell therapy.