Transcriptome analysis of an oil-rich race B strain of Botryococcus braunii (BOT-22) by de novo assembly of pyrosequencing cDNA reads

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• 作者：Motohide Ioki^a ; ¹ ; Masato Baba^b ; ¹ ; Nobuyoshi Nakajima^a ; ^b ; ^{naka-320@nies.go.jp} ; Yoshihiro Shiraiwa^b ; Makoto M. Watanabe^b
• 关键词：Botryococcus braunii ; Expressed sequence tag (EST) ; Hydrocarbon ; Pyrosequencing ; Triterpenoid
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：April, 2012
• 卷：109
• 期：Complete
• 页码：292-296
• 文件大小：251 K

摘要

To gain genetic insights into the biosynthesis of botryococcene oils in Botryococcus braunii race B, a transcriptome dataset of the BOT-22 strain containing 27,427 non-redundant sequences assembled from 209,429 complementary DNA reads was obtained via high-throughput 454 sequencing. Relatively reliable prediction of the gene product was feasible for 725 non-redundant sequences based on homology to previously characterized database sequences. Regarding the botryococcene oil biosynthesis, genes putatively associated with the mevalonate-independent isoprenoid biosynthesis pathway were retrieved, while no genes were found for the mevalonate pathway, suggesting that botryococcenes are biosynthesized through the mevalonate-independent pathway in B. braunii. All transcriptome sequences have been deposited in the GenBank/EMBL/DDBJ database.