TBP recruitment to the U1 snRNA gene promoter is disrupted by substituting a U6 proximal sequence element A (PSEA) for the U1 PSEA
- 作者：Nermeen H. Barakat ; William E. Stumph
- 关键词：snRNA genes ; Pre-initiation complex assembly ; SNAPc ; TBP ; RNA polymerase specificity
- 刊名：FEBS Letters
- 出版年：2008
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：9 July 2008
- 卷：582
- 期：16
- 页码：2413-2416
- 文件大小：241 K
摘要
Transcription of Drosophila U1 or U6 snRNAs by RNA polymerases II and III respectively requires a unique 
om/scidirimg/entities/223c.gif" alt="not, vert, similar" title="not, vert, similar" border="0">21 base-pair promoter element termed the proximal sequence element A (PSEA) recognized by the snRNA activating protein complex (DmSNAPc). A five-nucleotide substitution that changed the U1 PSEA to a U6 PSEA inactivated the U1 promoter. Chromatin immunoprecipitation assays indicated this substitution did not affect DmSNAPc DNA binding but instead interfered with SNAPc recruitment of TBP to the TATA-less U1 promoter. These findings support a model wherein sequence differences between the U1 and U6 PSEAs induce distinct DmSNAPc conformational states involved in RNA polymerase selectivity.