Quantitation of hepatitis B surface antigen by an automated chemiluminescent microparticle immunoassay
- 作者：Deguchi ; Matsuo ; Yamashita ; Naoko ; Kagita ; Masanori ; Asari ; Seishi ; Iwatani ; Yoshinori ; Tsuchida ; Takahiko ; et. al.
- 关键词：HBsAg ; HBsAg quantitation ; HBV-DNA ; HBV-DNA polymerase ; Architect ; CLIA
- 刊名：Journal of Virological Methods
- 出版年：2004
- 期刊代码：180_01660934
- 类别：med
- 出版时间：February, 2004
- 卷：115
- 期：2
- 页码：217-222
- 文件大小：165 K
摘要
A fully automated chemiluminescent microparticle immunoassay (Architect HBsAg QT) was used for the detection and quantitation of hepatitis B surface antigen (HBsAg). The assay is capable of processing up to 800 HBsAg tests per hour. The concentration of HBsAg is determined by utilizing a previously generated Architect HBsAg calibration curve. Architect HBsAg QT sensitivity was found to be around 0.2ng/ml which is equivalent or superior to other known and commercially available enzyme immunoassays and/or chemiluminescent immunoassays. We performed a quantitative study of HBsAg, HBeAg, HBV-DNA and HBV-DNA polymerase in over 733 sera obtained from 43 chronic hepatitis B carriers. Serum HBsAg levels detected by Architect HBsAg QT were found to be higher in HBeAg-positive than in anti-HBe-positive HBV chronic carriers and correlated with the level of serum HBV-DNA and HBV-DNA polymerase.