Antimicrobial and antibiofilm action of Casbane Diterpene from Croton nepetaefolius against oral bacteria

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• 作者：Nairley Cardoso Sá ; ^a ; Theodora Thays Arruda Cavalcante^b ; Amanda Ximenes Araú ; jo^c ; Hé ; lcio Silva dos Santos^c ; Maria Rose Jane Ribeiro Albuquerque^c ; Paulo Nogueira Bandeira^c ; Rodrigo Maranguape Silva da Cunha^d ; Benildo Sousa Cavada^b ; Edson Holanda Teixeira^a ; ^edson@ufc.br
• 关键词：Casbane Diterpene ; Biofilm ; Oral bacteria
• 刊名：Archives of Oral Biology
• 出版年：2012
• 期刊代码：25_00039969
• 类别：med
• 出版时间：May, 2012
• 卷：57
• 期：5
• 页码：550-555
• 文件大小：339 K

摘要

Objective

The antibacterial activity of Casbane Diterpene (CD) was evaluated in vitro against Streptococcus oralis, S. mutans, S. salivarius, S. sobrinus, S. mitis and S. sanguinis. The viability of planktonic cells was analysed by susceptibility tests (MIC and MBC) and antibiofilm action was assayed.

Methods

The minimal inhibitory and bactericidal concentrations (MIC and MBC) of oral Streptococcus were evaluated through microdilution tests. To assay antibiofilm activity, biofilms were generated on 96-wells polystyrene plates under the presence of CD and quantified by a crystal violet technique and colonies forming units counting.

Results

The CD isolated from Croton nepetaefolius showed antimicrobial effect on planktonic forms and biofilms of oral pathogens, with MIC values of 62.5 渭g/mL against Streptococcus oralis and values between 125 and 500 渭g/mL against S. mutans, S. salivarius, S. sobrinus, S. mitis and S. sanguinis. CD showed an inhibitory effect on S. mutans biofilm formation at 250 渭g/mL, and a decrease on viable cell of 94.28%compared to the normal biofilm growth.

Conclusions

The compound CD can be considered as a promising molecule for the treatment against oral pathogens responsible for dental biofilm.