The identification of a VDAC-like protein involved in the interaction of Babesia bigemina sexual stages with Rhipicephalus microplus midgut cells

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• 作者：Elba Rodrí ; guez-Herná ; ndez^a ; Juan Mosqueda^b ; Marí ; a Elizbeth Alvarez-Sá ; nchez^a ; Alfonso Falcó ; n Neri^c ; Guillermo Mendoza-Herná ; ndez^d ; Minerva Camacho-Nuez^a ; ^{mcamachonuez@yahoo.com.mx}
• 关键词：Rhipicephalus microplus ; VDAC ; Babesia bigemina ; Midgut cells ; Apoptosis
• 刊名：Veterinary Parasitology
• 出版年：2012
• 期刊代码：107_03044017
• 类别：abs
• 出版时间：6 July, 2012
• 卷：187
• 期：3-4
• 页码：538-541
• 文件大小：670 K

摘要

We investigated the interaction of Rhipicephalus microplus midgut cells with Babesia bigemina sexual stages using a proteomic approach. A polypeptide from the R. microplus midgut that binds to proteins from B. bigemina sexual stages was identified and sequenced. Combining 2D overlay and tandem mass spectrometry (MS/MS) techniques, we determined that this polypeptide corresponds to a mitochondrial voltage-dependent anion-selective channel (VDAC). The vdac gene encoding the sequenced polypeptide was identified and sequenced. This is the first report of a VDAC-like protein in R. microplus, and a possible role for this protein in the B. bigemina infection process is suggested.