A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.)

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• 作者：Tsung-Meng Wu ; Ke-Chun Lin ; Wei-Shiang Liau ; Yun-Yang Chao…
• 关键词：Organelle marker lines ; GFP ; DsRed ; Oryza sativa ; Subcellular localization ; Protoplast
• 刊名：Plant Molecular Biology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：90
• 期：1-2
• 页码：107-115
• 全文大小：1,516 KB
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• 作者单位：Tsung-Meng Wu (1) (3)
  Ke-Chun Lin (1)
  Wei-Shiang Liau (1)
  Yun-Yang Chao (1) (4)
  Ling-Hung Yang (1)
  Szu-Yun Chen (1)
  Chung-An Lu (2)
  Chwan-Yang Hong (1)
  
  1. Department of Agricultural Chemistry, College of Bioresources and Agriculture, National Taiwan University, Taipei, 10617, Taiwan, ROC
  3. Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, ROC
  4. Department of Plant Industry, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, ROC
  2. Department of Life Sciences, National Central University, Jhongli City, Taoyuan County, 320, Taiwan, ROC
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Biochemistry
  Plant Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-5028

文摘

In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable fluorescent organelle marker lines are lacking in the monocot model rice. Here, we developed eight different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker lines. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and successfully co-localized with mitochondrial-targeted marker lines verified the practical use of this system. The co-localization of GFP-fusion marker lines and DsRed-fusion proteins provide a convenient platform for in vivo or in vitro analysis of subcellular localization of rice proteins.