Effects of Fusarium oxysporum on rhizosphere microbial communities of two cucumber genotypes with contrasting Fusarium wilt resistance under hydroponic condition
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- 作者：Ju Ding (1) (2)
Yiqing Zhang (1)
Huan Zhang (1)
Xin Li (1)
Zenghui Sun (1)
Yangwenke Liao (1)
Xiaojian Xia (1)
Yanhong Zhou (1)
Kai Shi (1)
Jingquan Yu (1) (3) - 关键词：Microbial community ; Root exudates ; Cucumis sativus ; Fusarium oxysporum ; Resistance ; Hydroponic system
- 刊名：European Journal of Plant Pathology
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：140
- 期：4
- 页码：643-653
- 全文大小：492 KB
- 参考文献：1. Alexander, L., Wolfgang, B., & Harald, M. (2005). Diversity of bacteria growing in natural mineral water after bottling. / Applied and Environmental Microbiology, 71, 3624鈥?632. CrossRef
2. Arif, M., Zaidi, N. W., Haq, Q. M. R., Singh, Y. P., Khan, S., & Singh, U. S. (2013). Molecular phylogeny and pathotyping of / Fusarium solani: a causal agent of / Dalbergia sissoo decline. / Forest Pathology, 43, 478鈥?87. CrossRef
3. Bais, H. P., Prithiviraj, B., Jha, A. K., Ausubel, F. M., & Vivanco, J. M. (2005). Mediation of pathogen resistance by exudation of antimicrobials from roots. / Nature, 434, 217鈥?21. CrossRef
4. Bais, H. P., Weir, T. L., Perry, L. G., Gilroy, S., & Vivanco, J. M. (2006). The role of root exudates in rhizosphere interactions with plants and other organisms. / Annual Review of Plant Biology, 57, 233鈥?66. CrossRef
5. Bernardet, J. F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K., & Vandamme, P. (1996). Cutting a Gordian knot: emended classification and description of the genus / Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of / Flavobacterium hydatis nom. / International Journal of Systematic Bacteriology, 46, 128鈥?48. CrossRef
6. Bulluck, L. R., III, & Ristaino, J. B. (2002). Effect of synthetic and organic soil fertility amendments on southern blight, soil microbial communities and yield of processing tomatoes. / Phytopathology, 92, 181鈥?89. CrossRef
7. Chisholm, S. T., Coaker, G., Day, B., & Staskawicz, B. J. (2006). Host鈥搈icrobe interactions: shaping the evolution of the plant immune response. / Cell, 124, 803鈥?14. CrossRef
8. Costa, R., G枚tz, M., Mrotzek, N., Lottmann, J., Berg, G., & Smalla, K. (2006). Effects of site and plant species on rhizosphere community structure as revealed by molecular analysis of microbial guilds. / FEMS Microbiology Ecology, 56, 236鈥?49. CrossRef
9. Dardanelli, S. M., de C贸rdoba, F. J. F., Est茅vez, J., Contreras, R., Cubo, M. T., Rodriguez-Carvajal, M. A., et al. (2012). Changes in flavonoids secreted by / Phaseolus vulgaris roots in the presence of salt and the plant growth-promoting rhizobacterium / Chryseobacterium balustinum. Applied Soil Ecology, 57, 31鈥?8. CrossRef
10. Deboy, R. T., Mongodin, E. F., Fouts, D. E., Tailford, L. E., Khouri, H., Emerson, J. B., et al. (2008). Insights into plant cell wall degradation from the genome sequence of the soil bacterium / Cellvibrio japonicus. Journal of Bacteriology, 190, 5455鈥?463. CrossRef
11. Elliott, L. F., & Lynch, J. M. (1994). Biodiversity and soil resilience. In D. J. Greenland & I. Szabolca (Eds.), / Soil resilience and sustainable land use (pp. 353鈥?64). Oxon: CAB International, Wallingford.
12. Flores, H. E., Vivanco, J. M., & Loyola-Vargas, V. M. (1999). 鈥淩adicle鈥?biochemistry: the biology of root-specific metabolism. / Trends in Plant Science, 4, 220鈥?26. CrossRef
13. Green, S. J., Michel, F. C., Hadar, J., & Minz, D. (2004). Similarity of bacterial communities in sawdust- and straw-amended cow manure composts. / FEMS Microbiology Letters, 233, 115鈥?23. CrossRef
14. Hartmann, A., Schmid, M., van Tuinen, D., & Berg, G. (2009). Plant-driven selection of microbes. / Plant and Soil, 321, 235鈥?57. CrossRef
15. He, C. Y., Hsiang, T., & Wolyn, D. J. (2002). Induction of systemic disease resistance and pathogen defence responses in / Asparagus offcinalis inoculated with nonpathogenic strains of / Fusarium oxysporum. Plant Pathology, 51, 225鈥?30. CrossRef
16. Inbar, E., Green, S. J., Hadar, Y., & Minz, D. (2005). Competing factors of compost concentration and proximity to root affect the distribution of streptomycetes. / Microbial Ecology, 50, 73鈥?1. CrossRef
17. Janvier, C., Villeneuve, F., Alabouvette, C., Edel-Hermann, V., Mateille, T., & Steinberg, C. (2007). Soil health through soil disease suppression: which strategy from descriptors to indicators? / Soil Biology and Biochemistry, 39, 1鈥?3. CrossRef
18. Kamilova, F., Kravchenko, L. V., Shaposhnikov, A. I., Makarova, N., & Lugtenberg, B. (2006). Effects of the tomato pathogen / Fusarium oxysporum f. sp. / radicis- / lycopersici and of the biocontrol bacterium / Pseudomonas fluorescens WCS365 on the composition of organic acids and sugars in tomato root exudates. / Molecular Plant-Microbe Interactions, 19, 1121鈥?126. CrossRef
19. Katsifas, E. A., Koraki, T. G., & Karagouni, A. D. (2000). Determination of metabolic activity of streptomycetes in soil microcosms. / Journal of Applied Microbiology, 89, 178鈥?84. CrossRef
20. Kavroulakis, N., Ntougias, S., Zervakis, G. I., Ehaliotis, C., Haralampidis, K., & Papadopoulou, K. K. (2007). Role of ethylene in the protection of tomato plants against soil-borne fungal pathogens conferred by an endophytic / Fusarium solani strain. / Journal of Experimental Botany, 58, 3853鈥?864. CrossRef
21. Khalil, S., & Alsanius, B. W. (2001). Dynamics of the indigenous microflora inhabiting the root zone and the nutrient solution of tomato in a commercial closed greenhouse system. / Gartenbauwissenschaft, 66, S.188鈥?98.
22. Komada, H. (1975). Development of a selective medium for quantitative isolation of / Fusarium oxysporum from natural soil. / Review of Plant Protection Research, 8, 115鈥?25.
23. Kuske, C. R., Ticknor, L. O., Miller, M. E., Dunbar, J. M., Davis, J. A., Barns, S. M., et al. (2002). Comparison of soil bacterial communities in rhizospheres of three plant species and the interspaces in an arid grassland. / Applied and Environmental Microbiology, 68, 1854鈥?863. CrossRef
24. Li, J. M., Jin, Z. X., & Gu, Q. P. (2011). Effect of plant species on the function and structure of the bacterial community in the rhizosphere of lead-zinc mine tailings in Zhejiang, China. / Canadian Journal of Microbiology, 57, 569鈥?77. CrossRef
25. Mark, G. L., Dow, J. M., Kiely, P. D., Higgins, H., Haynes, J., Baysse, C., et al. (2005). Transcriptome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions. / Proceedings of the National Academy of Sciences USA, 102, 17454鈥?7459. CrossRef
26. McCracken, V. J., Simpson, J. M., Mackie, R. I., & Gaskins, H. R. (2001). Molecular ecological analysis of dietary and antibiotic-induced alterations of the mouse intestinal microbiota. / The Journal of Nutrition, 131, 1862鈥?870.
27. Micallef, S. A., Channer, S., Shiaris, M. P., & Colon-Carmona, A. (2009). Plant age and genotype impact the progression of bacterial community succession in the / Arabidopsis rhizosphere. / Plant Signaling & Behavior, 4, 777鈥?80. CrossRef
28. Mitter, B., Brader, G., Afzal, M., Compant, S., Naveed, M., Trognitz, F., et al. (2013). Advances in elucidating beneficial interactions between plants, soil, and bacteria. In D. L. Sparks (Ed.), / Advances in agronomy (pp. 381鈥?45). Burlington: Academic.
29. Muyzer, G., de Waal, E. C., & Uitterlinden, A. G. (1993). Profiling of complex microbial population by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. / Applied and Environmental Microbiology, 59, 695鈥?00.
30. Nico, M., Ribaudo, C. M., Gori, J. I., Cantore, M. L., & Cura, J. A. (2012). Uptake of phosphate and promotion of vegetative growth in glucose-exuding rice plants ( / Oryza sativa) inoculated with plant growth-promoting bacteria. / Applied Soil Ecology, 61, 190鈥?95. CrossRef
31. N贸brega, F. M., Santos, I. S., Da Cunha, M., Carvalho, A. O., & Gomes, V. M. (2005). Antimicrobial proteins from cowpea root exudates: inhibitory activity against / Fusarium oxysporum and purification of a chitinase-like protein. / Plant and Soil, 272, 223鈥?32. CrossRef
32. Pankhurst, C. E., McDonald, H. J., Hawke, B. G., & Kirkby, C. A. (2002). Effect of tillage and stubble management on chemical and microbiological properties and the development of suppression towards cereal root disease in soil from two sites in NSW, Australia. / Soil Biology and Biochemistry, 34, 833鈥?40. CrossRef
33. Peng, H. X., Sivasithamparam, K., & Turner, D. W. (1999). Chlamydospore germination and Fusarium wilt of banana plantlets in suppressive and conducive soils are affected by physical and chemical factors. / Soil Biology and Biochemistry, 31, 1363鈥?374. CrossRef
34. Postma, J., Geraats, B. P. J., Pastoor, R., & van Elsas, J. D. (2005). Characterization of the microbial community involved in the suppression of Pythium aphanidermatum in cucumber grown on rockwool. / Phytopathology, 95, 808鈥?18. CrossRef
35. Sakellaris, H., Pemberton, J. M., & Manners, J. M. (1993). Characterization of an endo-1,3(4)-尾-glucanase from / Cellvibrio mixtus. FEMS Microbiology Letters, 109, 269鈥?72.
36. Schuhegger, R., Ihring, A., Gantner, S., Bahnweg, G., Knappe, C., Vogg, G., et al. (2006). Induction of systemic resistance in tomato by N-acyl-L-homoserine lactone-producing rhizosphere bacteria. / Plant, Cell and Environment, 29, 909鈥?18. CrossRef
37. Shannon, C. E., & Weaver, W. (1963). / The mathematical theory of communication. Urbana: University of Illinois Press.
38. Shidore, T., Dinse, T., Oehrlein, J., Becker, A., & Reinhold-Hurek, B. (2012). Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte / Azoarcus sp strain BH72. / Environmental Microbiology, 14, 2775鈥?787. CrossRef
39. Simpson, E. H. (1949). Measurement of diversity. / Nature, 163, 688. CrossRef
40. Tian, Q., Zhang, X., Gao, Y., Bai, W., Ge, F., Ma, Y., et al. (2013). Wheat genotypes differing in aluminum tolerance differ in their growth response to CO2 enrichment in acid soils. / Ecology and Evolution, 3, 1440鈥?448. CrossRef
41. van Elsas, J. D., Garbeva, P., & Salles, J. (2002). Effects of agronomical measures on the microbial diversity of soils as related to the suppression of soil-borne plant pathogens. / Biodegradation, 13, 29鈥?0. CrossRef
42. Wollum, A. G. (1982). Cultural methods for soil microorganisms. In A. L. Page (Ed.), / Methods of soil analysis part 2: chemical and microbial properties (pp. 781鈥?02). Madison: ASA, WI.
43. Yang, C. H., Crowley, D. E., & Menge, J. A. (2001). 16S rDNA fingerprinting of rhizosphere bacterial communities associated with healthy and / Phytophthora infected avocado roots. / FEMS Microbiology Ecology, 35, 129鈥?36. CrossRef
44. Yang, Q. X., Zhang, J., Zhang, W. Y., Wang, Z., Xie, Y. S., & Zhang, H. (2010). Influence of tetracycline exposure on the growth of wheat seedlings and the rhizosphere microbial community structure in hydroponic culture. / Journal of Environmental Science and Health. Part. B, 45, 190鈥?97. CrossRef
45. Ye, S. F., Yu, J. Q., Peng, Y. H., Zheng, J. H., & Zou, L. Y. (2004). Incidence of Fusarium wilt in / cucumis sativus L. is promoted by cinnamic acid, an autotoxin in root exudates. / Plant and Soil, 263, 143鈥?50. CrossRef
46. Yu, J. Q., & Komada, H. (1999). Hinoki bark, a substrate with antipathogen properties that suppress root diseases of tomato. / Scientia Horticulturae, 81, 13鈥?4. CrossRef
47. Yu, J. Q., & Matsui, Y. (1994). Phytotoxic substances in the root exudates of / Cucumis sativus L. / Journal of Chemical Ecology, 20, 21鈥?1. CrossRef
48. Zamioudis, C., & Pieterse, C. M. J. (2012). Modulation of host immunity by beneficial microbes. / Molecular Plant-Microbe Interactions, 25, 139鈥?50. CrossRef - 作者单位：Ju Ding (1) (2)
Yiqing Zhang (1)
Huan Zhang (1)
Xin Li (1)
Zenghui Sun (1)
Yangwenke Liao (1)
Xiaojian Xia (1)
Yanhong Zhou (1)
Kai Shi (1)
Jingquan Yu (1) (3)
1. Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, People鈥檚 Republic of China
2. Seed Management Station of Xiaoshan District, 1169 Gongxiu Road, Hangzhou, 311201, People鈥檚 Republic of China
3. Key Laboratory of Horticultural Plants Growth, Development and Quality Improvement, Agricultural Ministry of China, 866 Yuhangtang Road, Hangzhou, 310058, People鈥檚 Republic of China - ISSN：1573-8469
文摘
Two cucumber genotypes were chosen that differ in Fusarium wilt resistance and inoculated with Fusarium oxysporum (Schlechtend, Fr) f. sp. cucumerinum (Owen) Snyder & Hansen (FO) in a hydroponic nutrient solution system to determine whether differences in plant genotype affected rhizosphere microbial community and its involvement in resistance to root-borne pathogens. Response of the FO population and microbial communities were compared in the nutrient solution and on root surfaces. Results demonstrated that FO inoculation resulted in a higher FO population on root surfaces and a lower population in the nutrient solution for the susceptible genotype JinYan NO. 4 (JYan); however, an inverse pattern was observed in the resistant JinYou NO. 1 (JYou). Similarly, promotive effects on FO spore germination and germ-tube elongation were only observed in root exudates from FO-inoculated susceptible JYan. FO inoculation resulted in overall increases in cultivable fungi and oomycetes, and actinomycete populations on root surfaces of the resistant JYou compared to the JYan counterpart. PCR-DGGE analysis of 16S rDNA fragments indicated that following FO inoculation, significant changes in the bacterial structure isolated from the root surfaces of susceptible plants were observed, such as an increased diversity index, a decreased evenness index, and the occurrence of several types of bacteria that decomposed organic substances. These results suggest that a complex interaction between plant genotypes and pathogen affected the rhizosphere microbial community.