MiR-21 up-regulation mediates glioblastoma cancer stem cells apoptosis and proliferation by targeting FASLG

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• 作者：Chao Shang (1)
  Yan Guo (2)
  Yang Hong (3)
  Yun-hui Liu (3)
  Yi-xue Xue (1)
  
  1. Department of Neurobiology ; China Medical University ; No. 92 Beier Road ; Heping District ; Shenyang ; 110001 ; People鈥檚 Republic of China
  2. Department of Central Laboratory ; School of Stomatology ; China Medical University ; Shenyang ; 110007 ; People鈥檚 Republic of China
  3. Department of Neurosurgery ; Shengjing Hospital ; China Medical University ; Shenyang ; 110004 ; People鈥檚 Republic of China
  
• 关键词：Glioblastoma multiforme ; MiR ; 21 ; FASLG ; Cancer stem cells ; U87
• 刊名：Molecular Biology Reports
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：42
• 期：3
• 页码：721-727
• 全文大小：887 KB
• 参考文献：1. Kuhnt D, Becker A, Ganslandt O, Bauer M, Buchfelder M, Nimsky C (2011) Correlation of the extent of tumor volume resection and patient survival in surgery of glioblastoma multiforme with high-field intraoperative MRI guidance. Neuro Oncol 13(12):1339鈥?348 CrossRef
  2. Wang S, Huang S, Zhao X, Zhang Q, Wu M, Sun F, Han G, Wu D (2013) Enrichment of prostate cancer stem cells from primary prostate cancer cultures of biopsy samples. Int J Clin Exp Pathol 7(1):184鈥?93
  3. Morihiro Y, Yasumoto Y, Vaidyan LK, Sadahiro H, Uchida T, Inamura A, Sharifi K, Ideguchi M, Nomura S, Tokuda N, Kashiwabara S, Ishii A, Ikeda E, Owada Y, Suzuki M (2013) Fatty acid binding protein 7 as a marker of glioma stem cells. Pathol Int 63(11):546鈥?53 CrossRef
  4. Sato A, Okada M, Shibuya K, Watanabe E, Seino S, Narita Y, Shibui S, Kayama T, Kitanaka C (2014) Pivotal role for ROS activation of p38 MAPK in the control of differentiation and tumor-initiating capacity of glioma-initiating cells. Stem Cell Res 12(1):119鈥?31 CrossRef
  5. Wu L, Li G, Feng D, Qin H, Gong L, Zhang J, Zhang Z (2013) MicroRNA-21 expression is associated with overall survival in patients with glioma. Diagn Pathol 8(1):200 CrossRef
  6. Xiong B, Cheng Y, Ma L, Zhang C (2013) MiR-21 regulates biological behavior through the PTEN/PI-3K/Akt signaling pathway in human colorectal cancer cells. Int J Oncol 42(1):219鈥?28
  7. Zhang W, Bai W, Zhang W (2013) MiR-21 suppresses the anticancer activities of curcumin by targeting PTEN gene in human non-small cell lung cancer A549 cells. Clin Transl Oncol 16(8):708鈥?13
  8. Kawakita A, Yanamoto S, Yamada SI, Naruse T, Takahashi H, Kawasaki G, Umeda M (2013) MicroRNA-21 promotes oral cancer invasion via the Wnt/尾-catenin pathway by targeting DKK2. Pathol Oncol Res 20:253鈥?61 CrossRef
  9. Rich JN, Eyler CE (2008) Cancer stem cells in brain tumor biology. Cold Spring Harb Symp Quant Biol 73:411鈥?20 CrossRef
  10. Jin X, Jin X, Jung JE, Beck S, Kim H (2013) Cell surface Nestin is a biomarker for glioma stem cells. Biochem Biophys Res Commun 433(4):496鈥?01 CrossRef
  11. Sato A, Okada M, Shibuya K, Watanabe E, Seino S, Narita Y, Shibui S, Kayama T, Kitanaka C (2014) Pivotal role for ROS activation of p38 MAPK in the control of differentiation and tumor-initiating capacity of glioma-initiating cells. Stem Cell Res 12(1):119鈥?31 CrossRef
  12. Sampetrean O, Saya H (2013) Characteristics of glioma stem cells. Brain Tumor Pathol 30(4):209鈥?14 CrossRef
  13. Lim HY, Kim KM, Kim BK, Shim JK, Lee JH, Huh YM, Kim SH, Kim EH, Park EK, Shim KW, Chang JH, Kim DS, Kim SH, Hong YK, Lee SJ, Kang SG (2013) Isolation of mesenchymal stem-like cells in meningioma specimens. Int J Oncol 43(4):1260鈥?268
  14. Vescovi AL, Galli R, Reynolds BA (2006) Brain tumour stem cells. Nat Rev Cancer 6(6):425鈥?36 CrossRef
  15. Hong X, Chedid K, Kalkanis SN (2012) Glioblastoma cell line-derived spheres in serum-containing medium versus serum-free medium: a comparison of cancer stem cell properties. Int J Oncol 41(5):1693鈥?700
  16. Li M, Li J, Liu L, Li W, Yang Y, Yuan J (2013) MicroRNA in Human Glioma. Cancers (Basel). 5(4):1306鈥?331 CrossRef
  17. Tu Y, Gao X, Li G, Fu H, Cui D, Liu H, Jin W, Zhang Y (2013) MicroRNA-218 inhibits glioma invasion, migration, proliferation, and cancer stem-like cell self-renewal by targeting the polycomb group gene Bmi1. Cancer Res 73(19):6046鈥?055 CrossRef
  18. Oh JS, Kim JJ, Byun JY, Kim IA (2010) Lin28-let7 modulates radiosensitivity of human cancer cells with activation of K-Ras. Int J Radiat Oncol Biol Phys 76(1):5鈥? CrossRef
  19. Shen J, Wan R, Hu G, Yang L, Xiong J, Wang F, Shen J, He S, Guo X, Ni J, Guo C, Wang X (2012) miR-15b and miR-16 induce the apoptosis of rat activated pancreatic stellate cells by targeting Bcl-2 in vitro. Pancreatology 12(2):91鈥?9 CrossRef
  20. Yao Y, Li L, Huang X, Gu X, Xu Z, Zhang Y, Huang L, Li S, Dai Z, Li C, Zhou T, Cai W, Yang Z, Gao G, Yang X (2013) SERPINA3聽K induces apoptosis in human colorectal cancer cells via activating the Fas/FasL/caspase-8 signaling pathway. FEBS J 280(14):3244鈥?255 CrossRef
  21. Liu WH, Chang LS (2011) Fas/FasL-dependent and -independent activation of caspase-8 in doxorubicin-treated human breast cancer MCF-7 cells: ADAM10 down-regulation activates Fas/FasL signaling pathway. Int J Biochem Cell Biol 43(12):1708鈥?719 CrossRef
  22. Wang WZ, Li L, Liu MY, Jin XB, Mao JW, Pu QH, Meng MJ, Chen XG, Zhu JY (2013) Curcumin induces FasL-related apoptosis through p38 activation in human hepatocellular carcinoma Huh7 cells. Life Sci 92(6鈥?):352鈥?58
  23. Yao Y, Li L, Huang X, Gu X, Xu Z, Zhang Y, Huang L, Li S, Dai Z, Li C, Zhou T, Cai W, Yang Z, Gao G, Yang X (2013) SERPINA3K induces apoptosis in human colorectal cancer cells via activating the Fas/FasL/caspase-8 signaling pathway. FEBS J 280(14):3244鈥?255 CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

To investigate whether miR-21 can affect the apoptosis and proliferation of glioblastoma cancer stem cells (GSCs) from down-regulating FASLG. The expression of miRNA-21 was detected by quantitative real-time PCR in normal brain tissue and glioblastoma samples, and the changes of miRNA-21 expression between GSCs and non-GSCs were also detected. The apoptosis and proliferation ability of miR-21 in GSCs were analyzed by MTT and flow cytometry assay after anti-miR-21 transfection. For the regulation mechanism analysis of miR-21, TargetScan, PicTar and microRNA were selected to predict some potential target genes of miR-21. The predicted gene was identified to be the direct and specific target gene of miR-21 by luciferase activities assay and western blot. RNA interference technology was used to confirm the apoptosis and proliferation effects of miR-21 were directly induced by FASLG. The expression of miR-21 increased significantly in glioblastoma contrast to normal brain tissue, and miR-21 up-regulated in GSCs remarkably. The proliferation of GSCs cell could be inhibited with high-expression of miR-21 and this effect could be restored by miR-21 knocked down. Mechanism analysis revealed that FASLG was a specific and direct target gene of miR-21. The advanced effects of anti-miR-21 on GSCs apoptosis and proliferation were mediated by expression of silenced FASLG. In summary, aberrantly expressed miR-21 regulates GSCs apoptosis and proliferation partly through directly down-regulating FASLG protein expression in GSCs and this might offer a new potential therapeutic stratagem for glioblastoma.