Cloning a glutathione peroxidase gene from Nelumbo nucifera and enhanced salt tolerance by overexpressing in rice

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• 作者：Ying Diao (1) (2)
  Huaxue Xu (1)
  Guolin Li (1)
  Aiqing Yu (1)
  Xia Yu (1)
  Wanling Hu (3)
  Xingfei Zheng (1)
  Shaoqing Li (1)
  Youwei Wang (2)
  Zhongli Hu (1)
  
• 关键词：Nelumbo nucifera ; Glutathione peroxidase (GPX) ; Reactive oxygen species (ROS) ; Rice ; Transformation ; Salt stress
• 刊名：Molecular Biology Reports
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：41
• 期：8
• 页码：4919-4927
• 全文大小：1,544 KB
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• 作者单位：Ying Diao (1) (2)
  Huaxue Xu (1)
  Guolin Li (1)
  Aiqing Yu (1)
  Xia Yu (1)
  Wanling Hu (3)
  Xingfei Zheng (1)
  Shaoqing Li (1)
  Youwei Wang (2)
  Zhongli Hu (1)
  
  1. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China
  2. School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China
  3. Institute of WTO, Wuhan University, Wuhan, 430072, People’s Republic of China
  
• ISSN：1573-4978

文摘

A full-length cDNA clone encoding an 866?bp-length glutathione peroxidase protein (NnGPX) was isolated from lotus (Nelumbo nucifera L.). The deduced amino acid sequence of the NnGPX gene had significant homology with ATGPX6. A 3D structural model of the NnGPX was constructed by homology modeling. The cloned NnGPX gene was expressed in Escherichia coli, and a fusion protein of about 40?kDa was detected after isopropyl thiogalactoside induction. Under different concentrations of Na2SeO3 treatments, NnGPX was found to be an enzyme that does not contain selenium. Real-time PCR analysis showed that the NnGPX gene was expressed in all organs of lotus, and its high expression mainly occurred in organs with active metabolisms. NnGPX transcript increased remarkably in response to cold, heat, mechanical damage, and salt treatment. Subsequently, the NnGPX gene was introduced in Oryza sativa cv. Yuetai B. PCR results verified the integration of this gene into the genome of rice and reverse transcription-PCR verified that this gene had been expressed in transgenic rice. The transgenic plants were significantly more tolerant to salt stress compared with the wild-type.