Production of human pro-relaxin H2 in the yeast Pichia pastoris
详细信息 查看全文
- 作者：D. Cimini ; K. Della Corte ; R. Finamore ; L. Andreozzi ; A. Stellavato…
- 关键词：Pro ; relaxin ; Pro ; hormone ; Protein instability ; Pichia pastoris ; IMAC ; LCMS
- 刊名：BMC Biotechnology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：17
- 期：1
- 全文大小：1264KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Applied Microbiology; Biotechnology; Biochemical Engineering; Genetic Engineering; Plant Breeding/Biotechnology; Biomedical Engineering/Biotechnology;
- 出版者：BioMed Central
- ISSN：1472-6750
- 卷排序：17
文摘
BackgroundInitially known as the reproductive hormone, relaxin was shown to possess other therapeutically useful properties that include extracellular matrix remodeling, anti-inflammatory, anti-ischemic and angiogenic effects. All these findings make relaxin a potential drug for diverse medical applications. Its precursor, pro-relaxin, is an 18 kDa protein, that shows activity in in vitro assays. Since extraction of relaxin from animal tissues raises several issues, prokaryotes and eukaryotes were both used as expression systems for recombinant relaxin production. Most productive results were obtained when using Escherichia coli as a host for human relaxin expression. However, in such host, relaxin precipitated in the form of inclusion bodies and, therefore, required several expensive recovery steps as cell lysis, refolding and reduction.