Proteomic analysis of human osteoarthritis synovial fluid
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- 作者：Lavanya Balakrishnan (1) (2)
Raja Sekhar Nirujogi (1) (3)
Sartaj Ahmad (1) (4)
Mitali Bhattacharjee (1) (5)
Srikanth S Manda (1) (3)
Santosh Renuse (1) (5)
Dhanashree S Kelkar (1) (5)
Yashwanth Subbannayya (1) (6)
Rajesh Raju (1)
Renu Goel (1) (2)
Joji Kurian Thomas (1) (5)
Navjyot Kaur (7)
Mukesh Dhillon (7)
Shantal Gupta Tankala (7)
Ramesh Jois (8)
Vivek Vasdev (9)
YL Ramachandra (2)
Nandini A Sahasrabuddhe (1)
TS Keshava Prasad (1) (3) (4)
Sujatha Mohan (10)
Harsha Gowda (1)
Subramanian Shankar (7)
Akhilesh Pandey (11) (12) (13) (14) - 关键词：Body fluid ; Cartilage ; Joint destruction ; Glycosylation
- 刊名：Clinical Proteomics
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：11
- 期：1
- 全文大小：422 KB
- 参考文献：1. Goldring SR, Goldring MB:
2. Rousseau JC, Delmas PD:
3. Gobezie R, Kho A, Krastins B, Sarracino DA, Thornhill TS, Chase M, Millett PJ, Lee DM:
4. Longo D, Fauci A, Kasper D, Hauser S, Jameson J, Loscalzo J: / Harrison's Principles of Internal Medicine. 18th edition. New York: Mcgraw-hill; 2011.
5. Bhattacharyya T, Gale D, Dewire P, Totterman S, Gale ME, McLaughlin S, Einhorn TA, Felson DT:
6. De Ceuninck F, Berenbaum F:
7. Ruiz-Romero C, Blanco FJ:
8. Hui AY, McCarty WJ, Masuda K, Firestein GS, Sah RL:
9. Adachi J, Kumar C, Zhang Y, Olsen JV, Mann M:
10. Barbhuiya MA, Sahasrabuddhe NA, Pinto SM, Muthusamy B, Singh TD, Nanjappa V, Keerthikumar S, Delanghe B, Harsha HC, Chaerkady R, Jalaj V, Gupta S, Shrivastav BR, Tiwari PK, Pandey A:
11. de Souza GA, Godoy LM, Mann M:
12. Liu T, Qian WJ, Gritsenko MA, Xiao W, Moldawer LL, Kaushal A, Monroe ME, Varnum SM, Moore RJ, Purvine SO, Maier RV, Davis RW, Tompkins RG, Camp DG 2nd, Smith RD, Inflammation and the Host Response to Injury Large Scale Collaborative Research Programm:
13. Marimuthu A, O'Meally RN, Chaerkady R, Subbannayya Y, Nanjappa V, Kumar P, Kelkar DS, Pinto SM, Sharma R, Renuse S, Goel R, Christopher R, Delanghe B, Cole RN, Harsha HC, Pandey A:
14. Molina H, Bunkenborg J, Reddy GH, Muthusamy B, Scheel PJ, Pandey A:
15. Pilch B, Mann M:
16. Yamagiwa H, Sarkar G, Charlesworth MC, McCormick DJ, Bolander ME:
17. Kamphorst JJ, van der Heijden R, DeGroot J, Lafeber FP, Reijmers TH, van El B, Tjaden UR, van der Greef J, Hankemeier T:
18. Wang Q, Rozelle AL, Lepus CM, Scanzello CR, Song JJ, Larsen DM, Crish JF, Bebek G, Ritter SY, Lindstrom TM, Hwang I, Wong HH, Punzi L, Encarnacion A, Shamloo M, Goodman SB, Wyss-Coray T, Goldring SR, Banda NK, Thurman JM, Gobezie R, Crow MK, Holers VM, Lee DM, Robinson WH:
19. Sohn DH, Sokolove J, Sharpe O, Erhart JC, Chandra PE, Lahey LJ, Lindstrom TM, Hwang I, Boyer KA, Andriacchi TP, Robinson WH:
20. Ritter SY, Subbaiah R, Bebek G, Crish J, Scanzello CR, Krastins B, Sarracino D, Lopez MF, Crow MK, Aigner T, Goldring MB, Goldring SR, Lee DM, Gobezie R, Aliprantis AO:
21. de Seny D, Sharif M, Fillet M, Cobraiville G, Meuwis MA, Mar茅e R, Hauzeur JP, Wehenkel L, Louis E, Merville MP, Kirwan J, Ribbens C, Malaise M:
22. Keshava Prasad TS, Goel R, Kandasamy K, Keerthikumar S, Kumar S, Mathivanan S, Telikicherla D, Raju R, Shafreen B, Venugopal A, Balakrishnan L, Marimuthu A, Banerjee S, Somanathan DS, Sebastian A, Rani S, Ray S, Harrys Kishore CJ, Kanth S, Ahmed M, Kashyap MK, Mohmood R, Ramachandra YL, Krishna V, Rahiman BA, Mohan S, Ranganathan P, Ramabadran S, Chaerkady R, Pandey A:
23. Prasad TS, Kandasamy K, Pandey A:
24. Martel-Pelletier J, Boileau C, Pelletier JP, Roughley PJ:
25. Kiani C, Chen L, Wu YJ, Yee AJ, Yang BB:
26. El-Arman MM, El-Fayoumi G, El-Shal E, El-Boghdady I, El-Ghaweet A:
27. Li H, Wang D, Wu ZQ, Zhong JM, Yuan YJ:
28. Xie DL, Meyers R, Homandberg GA:
29. Tchetverikov I, Lohmander LS, Verzijl N, Huizinga TW, TeKoppele JM, Hanemaaijer R, DeGroot J:
30. Neu CP, Reddi AH, Komvopoulos K, Schmid TM, Di Cesare PE:
31. Hunter DJ, Felson DT:
32. Lorenzo P, Aspberg A, Onnerfjord P, Bayliss MT, Neame PJ, Heinegard D:
33. Sakao K, Takahashi KA, Arai Y, Saito M, Honjyo K, Hiraoka N, Kishida T, Mazda O, Imanishi J, Kubo T:
34. Duval E, Bigot N, Hervieu M, Kou I, Leclercq S, Galera P, Boumediene K, Bauge C:
35. Kalamajski S, Aspberg A, Lindblom K, Heinegard D, Oldberg A:
36. Kizawa H, Kou I, Iida A, Sudo A, Miyamoto Y, Fukuda A, Mabuchi A, Kotani A, Kawakami A, Yamamoto S, Uchida A, Nakamura K, Notoya K, Nakamura Y, Ikegawa S:
37. Nakajima M, Kizawa H, Saitoh M, Kou I, Miyazono K, Ikegawa S:
38. Kohfeldt E, Sasaki T, Gohring W, Timpl R:
39. Kruegel J, Sadowski B, Miosge N:
40. Shapiro LH, Ashmun RA, Roberts WM, Look AT:
41. Fukasawa K, Fujii H, Saitoh Y, Koizumi K, Aozuka Y, Sekine K, Yamada M, Saiki I, Nishikawa K:
42. Lendeckel U, Arndt M, Bukowska A, Tadje J, Wolke C, Kahne T, Neubert K, Faust J, Ittenson A, Ansorge S, Reinhold D:
43. Mina-Osorio P, Winnicka B, O'Conor C, Grant CL, Vogel LK, Rodriguez-Pinto D, Holmes KV, Ortega E, Shapiro LH:
44. Bates EE, Fridman WH, Mueller CG:
45. Fritsche J, Muller A, Hausmann M, Rogler G, Andreesen R, Kreutz M:
46. Hu B, Coulson L, Moyer B, Price PA:
47. Price PA, Nguyen TM, Williamson MK:
48. Brochmann EJ, Simon RJ, Jawien J, Behnam K, Sintuu C, Wang JC, Murray SS:
49. Sintuu C, Murray SS, Behnam K, Simon R, Jawien J, Silva JD, Duarte ME, Brochmann EJ:
50. Agarwal SK, Brenner MB:
51. Shur I, Socher R, Hameiri M, Fried A, Benayahu D:
52. Sato-Nishiuchi R, Nakano I, Ozawa A, Sato Y, Takeichi M, Kiyozumi D, Yamazaki K, Yasunaga T, Futaki S, Sekiguchi K:
53. Sommarin Y, Wendel M, Shen Z, Hellman U, Heinegard D:
54. Buchaille R, Couble ML, Magloire H, Bleicher F:
55. Rehn AP, Cerny R, Sugars RV, Kaukua N, Wendel M:
56. Balint E, Lapointe D, Drissi H, van der Meijden C, Young DW, van Wijnen AJ, Stein JL, Stein GS, Lian JB:
57. Ducy P, Desbois C, Boyce B, Pinero G, Story B, Dunstan C, Smith E, Bonadio J, Goldstein S, Gundberg C, Bradley A, Karsenty G:
58. Hamajima S, Hiratsuka K, Kiyama-Kishikawa M, Tagawa T, Kawahara M, Ohta M, Sasahara H, Abiko Y:
59. Balint G, Barabas K, Zeitler Z, Bakos J, Kekesi KA, Pethes A, Nagy E, Lakatos T, Balint PV, Szekanecz Z:
60. Zhu Y, Xu G, Patel A, McLaughlin MM, Silverman C, Knecht K, Sweitzer S, Li X, McDonnell P, Mirabile R, Zimmerman D, Boyce R, Tierney LA, Hu E, Livi GP, Wolf B, Abdel-Meguid SS, Rose GD, Aurora R, Hensley P, Briggs M, Young PR:
61. Zhang LS, Hu HG, Liu YJ, Li J, Yu P, Zhang F, Yang TL, Tian Q, Zheng YP, Guo Y, Deng HW:
62. Zheng HF, Tobias JH, Duncan E, Evans DM, Eriksson J, Paternoster L, Yerges-Armstrong LM, Lehtim盲ki T, Bergstr枚m U, K盲h枚nen M, Leo PJ, Raitakari O, Laaksonen M, Nicholson GC, Viikari J, Ladouceur M, Lyytik盲inen LP, Medina-Gomez C, Rivadeneira F, Prince RL, Sievanen H, Leslie WD, Mellstr枚m D, Eisman JA, Mov茅rare-Skrtic S, Goltzman D, Hanley DA, Jones G, St Pourcain B, Xiao Y, Timpson NJ, Smith GD, Reid IR, Ring SM, Sambrook PN, Karlsson M, Dennison EM, Kemp JP, Danoy P, Sayers A, Wilson SG, Nethander M, McCloskey E, Vandenput L, Eastell R, Liu J, Spector T, Mitchell BD, Streeten EA, Brommage R, Pettersson-Kymmer U, Brown MA, Ohlsson C, Richards JB, Lorentzon M:
63. Hwang HJ, Quinn T, Zhang J:
64. Jerkovic L, Voegele AF, Chwatal S, Kronenberg F, Radcliffe CM, Wormald MR, Lobentanz EM, Ezeh B, Eller P, Dejori N, Dieplinger B, Lottspeich F, Sattler W, Uhr M, Mechtler K, Dwek RA, Rudd PM, Baier G, Dieplinger H:
65. Kim BJ, Lee YS, Lee SY, Park SY, Dieplinger H, Ryu SH, Yea K, Choi S, Lee SH, Koh JM, Kim GS:
66. Cattano NM, Driban JB, Balasubramanian E, Barbe MF, Amin M, Sitler MR:
67. Nielsen BB, Kastrup JS, Rasmussen H, Holtet TL, Graversen JH, Etzerodt M, Thogersen HC, Larsen IK:
68. Karlsson C, Dehne T, Lindahl A, Brittberg M, Pruss A, Sittinger M, Ringe J:
69. Loeser RF, Olex AL, McNulty MA, Carlson CS, Callahan MF, Ferguson CM, Chou J, Leng X, Fetrow JS:
70. Xiao SM, Gao Y, Cheung CL, Bow CH, Lau KS, Sham PC, Tan KC, Kung AW:
71. Meng J, Ma X, Ma D, Xu C:
72. Kandasamy K, Keerthikumar S, Goel R, Mathivanan S, Patankar N, Shafreen B, Renuse S, Pawar H, Ramachandra YL, Acharya PK, Ranganathan P, Chaerkady R, Keshava Prasad TS, Pandey A:
73. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ:
74. Harsha HC, Molina H, Pandey A:
75. Chaerkady R, Harsha HC, Nalli A, Gucek M, Vivekanandan P, Akhtar J, Cole RN, Simmers J, Schulick RD, Singh S, Torbenson M, Pandey A, Thuluvath PJ:
76. Rappsilber J, Mann M, Ishihama Y:
77. Olsen JV, de Godoy LM, Li G, Macek B, Mortensen P, Pesch R, Makarov A, Lange O, Horning S, Mann M:
78. Kandasamy K, Pandey A, Molina H:
79. MacLean B, Tomazela DM, Shulman N, Chambers M, Finney GL, Frewen B, Kern R, Tabb DL, Liebler DC, MacCoss MJ:
80. Holstein Sherwood CA, Gafken PR, Martin DB:
81. Paoletti AC, Parmely TJ, Tomomori-Sato C, Sato S, Zhu D, Conaway RC, Conaway JW, Florens L, Washburn MP:
82. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G: - 作者单位：Lavanya Balakrishnan (1) (2)
Raja Sekhar Nirujogi (1) (3)
Sartaj Ahmad (1) (4)
Mitali Bhattacharjee (1) (5)
Srikanth S Manda (1) (3)
Santosh Renuse (1) (5)
Dhanashree S Kelkar (1) (5)
Yashwanth Subbannayya (1) (6)
Rajesh Raju (1)
Renu Goel (1) (2)
Joji Kurian Thomas (1) (5)
Navjyot Kaur (7)
Mukesh Dhillon (7)
Shantal Gupta Tankala (7)
Ramesh Jois (8)
Vivek Vasdev (9)
YL Ramachandra (2)
Nandini A Sahasrabuddhe (1)
TS Keshava Prasad (1) (3) (4)
Sujatha Mohan (10)
Harsha Gowda (1)
Subramanian Shankar (7)
Akhilesh Pandey (11) (12) (13) (14)
1. Institute of Bioinformatics, International Technology Park, Bangalore, Karnataka, 560066, India
2. Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, Karnataka, 577451, India
3. Centre for Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Pondicherry, Puducherry, 605014, India
4. Manipal University, Madhava Nagar, Manipal, Karnataka, 576104, India
5. Amrita School of Biotechnology, Amrita University, Kollam, Kerala, 690525, India
6. Rajiv Gandhi University of Health Sciences, Bangalore, Karnataka, 560041, India
7. Department of Internal Medicine, Armed Forces Medical College, Pune, Maharashtra, 411040, India
8. Department of Rheumatology, Fortis Hospitals, Bangalore, Karnataka, 560076, India
9. Department of Rheumatology, Command Airforce Hospital, Bangalore, 560008, India
10. Laboratory for Integrated Bioinformatics, RIKEN Center for Integrative Medical Sciences (IMS-RCAI), Yokohama Institute, Yokohama, Kanagawa, 230-0045, Japan
11. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, 733 N. Broadway, BRB 527, Baltimore, MD, 21205, USA
12. Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
13. Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
14. Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA - ISSN：1559-0275
文摘
Background Osteoarthritis is a chronic musculoskeletal disorder characterized mainly by progressive degradation of the hyaline cartilage. Patients with osteoarthritis often postpone seeking medical help, which results in the diagnosis being made at an advanced stage of cartilage destruction. Sustained efforts are needed to identify specific markers that might help in early diagnosis, monitoring disease progression and in improving therapeutic outcomes. We employed a multipronged proteomic approach, which included multiple fractionation strategies followed by high resolution mass spectrometry analysis to explore the proteome of synovial fluid obtained from osteoarthritis patients. In addition to the total proteome, we also enriched glycoproteins from synovial fluid using lectin affinity chromatography. Results We identified 677 proteins from synovial fluid of patients with osteoarthritis of which 545 proteins have not been previously reported. These novel proteins included ADAM-like decysin 1 (ADAMDEC1), alanyl (membrane) aminopeptidase (ANPEP), CD84, fibulin 1 (FBLN1), matrix remodelling associated 5 (MXRA5), secreted phosphoprotein 2 (SPP2) and spondin 2 (SPON2). We identified 300 proteins using lectin affinity chromatography, including the glycoproteins afamin (AFM), attractin (ATRN), fibrillin 1 (FBN1), transferrin (TF), tissue inhibitor of metalloproteinase 1 (TIMP1) and vasorin (VSN). Gene ontology analysis confirmed that a majority of the identified proteins were extracellular and are mostly involved in cell communication and signaling. We also confirmed the expression of ANPEP, dickkopf WNT signaling pathway inhibitor 3 (DKK3) and osteoglycin (OGN) by multiple reaction monitoring (MRM) analysis of osteoarthritis synovial fluid samples. Conclusions We present an in-depth analysis of the synovial fluid proteome from patients with osteoarthritis. We believe that the catalog of proteins generated in this study will further enhance our knowledge regarding the pathophysiology of osteoarthritis and should assist in identifying better biomarkers for early diagnosis.