Development and characterization of twenty-two polymorphic microsatellite markers for the leafcutter ant, Acromyrmex lundii, utilizing Illumina sequencing

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• 作者：Christian Rabeling (1)
  Martin Bollazzi (2)
  Maurício Bacci Jr. (3)
  Rochelle R. Beasley (4)
  Stacey L. Lance (4)
  Kenneth L. Jones (5)
  Naomi E. Pierce (1)
  
• 关键词：Leafcutter ants ; Fungus ; growing ants ; Attini ; Acromyrmex ; Polygyny ; Polyandry ; Social parasitism ; Microsatellites ; Illumina ; PAL_FINDER ; PCR primers ; SSR
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：6
• 期：2
• 页码：319-322
• 全文大小：<len>
• 参考文献：1. Boomsma JJ, Fjerdingstad EJ, Frydenberg J (1999) Multiple paternity, relatedness and genetic diversity in / Acromyrmex leaf-cutter ants. Proc Royal Soc Lond Ser B Biol Sci 266:249-54 lass="external" href="http://dx.doi.org/10.1098/rspb.1999.0629" target="_blank" title="It opens in new window">CrossRef
  2. Castoe TA, Poole AW, de Koning AJ, Jones KL, Tomback DF, Oyler-McCance SJ, Fike JA, Lance SL, Streicher JW, Smith EN (2012) Rapid microsatellite identification from Illumina paired-end genomic sequencing in two birds and a snake. PLoS ONE 7:e30953 lass="external" href="http://dx.doi.org/10.1371/journal.pone.0030953" target="_blank" title="It opens in new window">CrossRef
  3. DeWoody JA, Schupp J, Kenefic L, Busch J, Murfitt L, Keim P (2004) Universal method for producing ROX-labeled size standards suitable for automated genotyping. Biotechniques 37:348-52
  4. Gon?alves CR (1961) O género / Acromyrmex no Brasil (Hym. Formicidae). Studia Entomologica 4:113-80
  5. Rabeling C, Bacci M (2010) A new workerless inquiline in the Lower Attini (Hymenoptera: Formicidae), with a discussion of social parasitism in fungus-growing ants. Syst Entomology 35:379-92 lass="external" href="http://dx.doi.org/10.1111/j.1365-3113.2010.00533.x" target="_blank" title="It opens in new window">CrossRef
  
• 作者单位：Christian Rabeling (1)
  Martin Bollazzi (2)
  Maurício Bacci Jr. (3)
  Rochelle R. Beasley (4)
  Stacey L. Lance (4)
  Kenneth L. Jones (5)
  Naomi E. Pierce (1)
  
  1. Museum of Comparative Zoology, Harvard University, 26 Oxford Street, Cambridge, MA, 02138, USA
  2. Section of Entomology, University of the Republic, Avenida Garzon 780, 11200, Montevideo, Uruguay
  3. Instituto de Biociências, Centro de Estudos de Insetos Sociais, UNESP, Universidade Estadual Paulista, Rio Claro, SP, 13506-900, Brazil
  4. Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, 29802, USA
  5. Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, 80045, USA
  
• ISSN：1877-7260

文摘

We isolated and characterized a total of 22 microsatellite loci for the leafcutter ant, Acromyrmex lundii. The loci were screened for 24 individuals from southern Brazil and Uruguay. The number of alleles per locus ranged from 5 to 20, the observed heterozygosity ranged from 0.417 to 0.917, and the probability of identity values ranged from 0.011 to 0.38. These genetic markers will be useful for understanding the population and conservation biology of the leafcutter ant A. lundii and closely related species, and will provide novel insights into the evolutionary biology of social parasitism and leafcutter ant mating systems.