Hex1-related transcriptome of Trichoderma atroviride reveals expression patterns of ABC transporters associated with tolerance to dichlorvos

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• 作者：Tailong Zhang ; Jun Tang ; Jianan Sun ; Chuanjin Yu ; Zhicheng Liu…
• 关键词：ABC transporter ; Dichlorvos ; Organophosphate pesticide ; RNA ; seq ; Tolerance ; Trichoderma
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：37
• 期：7
• 页码：1421-1429
• 全文大小：959 KB
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• 作者单位：Tailong Zhang (1) (2) (3)
  Jun Tang (4)
  Jianan Sun (1) (2) (3)
  Chuanjin Yu (1) (2) (3)
  Zhicheng Liu (1) (2) (3)
  Jie Chen (1) (2) (3)
  
  1. School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People’s Republic of China
  2. Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, People’s Republic of China
  3. State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, People’s Republic of China
  4. School of Life Science, Fuyang Teachers College, Fuyang, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Objectives The tolerance to organophosphate pesticide, dichlorvos, is essential for the application of Trichoderma in bioremediation and integrated pest management, although the molecular events associated with the tolerance process have not yet been elucidated. Results RNA-seq analysis of wild-type Trichoderma atroviride T23 and the hex1-deleted mutant under dichlorvos stress was designed to search for genes involved in the tolerance process. A total of 5382 differentially expressed genes were identified, highlighting the complex transcriptional changes of T. atroviride in response to dichlorvos stress. 137 genes were regulated by dichlorvos and hex1, encoding major facilitator superfamilies, cytochrome P450, glutathione-S-transferase, flavoprotein, Hsp70, Hsp90, etc. Pathway and expression analysis indicated that ABC transporters were affected by the disruption of hex1 gene and might play a vital role in the tolerance process. Expression patterns of seven selected ABC transporter genes were confirmed by qRT-PCR after exposure to dichlorvos for 2, 6 and 24?h. Conclusions The present study provides insights into the genetic basis of dichlorvos tolerance in Trichoderma that may be exploited for further development of bioremediation or biocontrol agents.