HOXA11 gene is hypermethylation and aberrant expression in gastric cancer
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- 作者：Yinguo Bai (1) (2)
Na Fang (2)
Tingxun Gu (2)
Yuhua Kang (1)
Jiang Wu (3)
Desheng Yang (1)
Hui Zhang (1)
Zhimin Suo (1)
Shaoping Ji (2)
1. Department of Gastroenterology ; Huaihe Hospital of Henan University ; Kaifeng ; Henan Province ; 475000 ; China
2. Department of Biochemistry and Molecular Biology ; Medical School of Henan University ; Kaifeng ; Henan Province ; 475004 ; China
3. Department of pathology ; Huaihe Hospital of Henan University ; Kaifeng ; Henan Province ; 475000 ; China - 关键词：Gastric cancer ; HOXA11 gene ; Methylation ; Expression
- 刊名：Cancer Cell International
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：14
- 期：1
- 全文大小：1,495 KB
- 参考文献：1. Pai, AA, Bell, JT, Marioni, JC, Pritchard, JK, Gilad, Y (2011) A genome-wide study of DNA methylation patterns and gene expression levels in multiple human and chimpanzee tissues[J]. PLoS Genet 7: pp. e1001316 nal" href="http://dx.doi.org/10.1371/journal.pgen.1001316" target="_blank" title="It opens in new window">CrossRef
2. Shirodkar, AV, Bernard, RS, Gavryushova, A, Kop, A, Knight, BJ, Yan, MS, Man, HSJ, Sud, M, Hebbel, RP, Oettgen, P, Aird, WC, Marsden, PA (2013) A mechanistic role for DNA methylation in endothelial cell (EC)-enriched gene expression: relationship with DNA replication timing[J]. Blood 121: pp. 3531-3540 nal" href="http://dx.doi.org/10.1182/blood-2013-01-479170" target="_blank" title="It opens in new window">CrossRef
3. Sugita, H, Iida, S, Inokuchi, M, Kato, K, Ishiguro, M, Ishikawa, T, Takagi, Y, Enjoji, M, Yamada, H, Uetake, H, Kojima, K, Sugihara, K (2011) Methylation of BNIP3 and DAPK indicates lower response to chemotherapy and poor prognosis in gastric cancer[J]. Oncol Rep 25: pp. 513-518 nal" href="http://dx.doi.org/10.3892/or.2010.1085" target="_blank" title="It opens in new window">CrossRef
4. Gao, Y, Baccarelli, A, Shu, XO, Ji, BT, Yu, K, Tarantini, L, Yang, G, Li, HL, Hou, L, Rothman, N, Zheng, W, Gao, YT, Chow, WH (2011) Blood leukocyte Alu and LINE-1 methylation and gastric cancer risk in the Shanghai Women鈥檚 Health Study[J]. Br J Cancer 106: pp. 585-591 nal" href="http://dx.doi.org/10.1038/bjc.2011.562" target="_blank" title="It opens in new window">CrossRef
5. Takamaru, H, Yamamoto, E, Suzuki, H, Nojima, M, Maruyama, R, Yamano, HO, Yoshikawa, K, Kimura, T, Harada, T, Ashida, M, Suzuki, R, Yamamoto, H, Kai, M, Tokino, T, Sugai, T, Imai, K, Toyota, M, Shinomura, Y (2012) Aberrant methylation of RASGRF1 is associated with an epigenetic field defect and increased risk of gastric cancer[J]. Cancer Prev Res 5: pp. 1203-1212 nal" href="http://dx.doi.org/10.1158/1940-6207.CAPR-12-0056" target="_blank" title="It opens in new window">CrossRef
6. Sun, M, Uozaki, H, Hino, R, Kunita, A, Shinozaki, A, Ushiku, T, Hibiya, T, Takeshita, K, Isogai, M, Takada, K, Fukayama, M (2012) SOX9 expression and its methylation status in gastric cancer[J]. Virchows Arch 460: pp. 271-279 nal" href="http://dx.doi.org/10.1007/s00428-012-1201-7" target="_blank" title="It opens in new window">CrossRef
7. Mallo, M, Alonso, CR (2013) The regulation of Hox gene expression during animal development[J]. Development 140: pp. 3951-3963 nal" href="http://dx.doi.org/10.1242/dev.068346" target="_blank" title="It opens in new window">CrossRef
8. Hur, H, Lee, JY, Yun, HJ, Park, BW, Kim, MH (2014) Analysis of HOX Gene Expression Patterns in Human Breast Cancer[J]. Mol Biotechnol 56: pp. 64-71 nal" href="http://dx.doi.org/10.1007/s12033-013-9682-4" target="_blank" title="It opens in new window">CrossRef
9. Dvorakova, E, Chmelarova, M, Laco, J, Palicka, V, Spacek, J (2013) Methylation analysis of tumor suppressor genes in endometroid carcinoma of endometrium using MS-MLPA[J]. Biomed Pap 157: pp. 298-303
10. Widschwendter, M, Apostolidou, S, Jones, AA, Fourkala, EO, Arora, R, Pearce, CL, Frasco, MA, Ayhan, A, Zikan, M, Cibula, D, Iyibozkurt, CA, Yavuz, E, Hauser-Kronberger, C, Dubeau, L, Menon, U, Jacobs, IJ (2009) HOXA methylation in normal endometrium from premenopausal women is associated with the presence of ovarian cancer: a proof of principle study[J]. International Journal of Cancer 125: pp. 2214-2218 nal" href="http://dx.doi.org/10.1002/ijc.24599" target="_blank" title="It opens in new window">CrossRef
11. Skiriut臈, D, Vaitkien臈, P, A拧monien臈, V, Steponaitis, G, Deltuva, VP, Tama拧auskas, A (2013) Promoter methylation of AREG, HOXA11, hMLH1, NDRG2, NPTX2 and Tes genes in glioblastoma[J]. J Neuro-Oncol 113: pp. 441-449 nal" href="http://dx.doi.org/10.1007/s11060-013-1133-3" target="_blank" title="It opens in new window">CrossRef
12. Apostolidou, S, Hadwin, R, Burnell, M, Jones, A, Baff, D, Pyndiah, N, Mould, T, Jacobs, IJ, Beddows, S, Kocjan, G, Widschwendter, M (2009) DNA methylation analysis in liquid鈥恇ased cytology for cervical cancer screening[J]. Int J Cancer 125: pp. 2995-3002 nal" href="http://dx.doi.org/10.1002/ijc.24745" target="_blank" title="It opens in new window">CrossRef
13. Tsang, DPF, Cheng, ASL (2011) Epigenetic regulation of signaling pathways in cancer: role of the histone methyltransferase EZH2[J]. J Gastroenterol Hepatol 26: pp. 19-27 nal" href="http://dx.doi.org/10.1111/j.1440-1746.2010.06447.x" target="_blank" title="It opens in new window">CrossRef
14. Kanwal, R, Gupta, S (2012) Epigenetic modifications in cancer[J]. Clin Genet 81: pp. 303-311 nal" href="http://dx.doi.org/10.1111/j.1399-0004.2011.01809.x" target="_blank" title="It opens in new window">CrossRef
15. Langevin, SM, Kelsey, KT (2013) The fate is not always written in the genes: Epigenomics in epidemiologic studies[J]. Environ Mol Mutagen 54: pp. 533-541 nal" href="http://dx.doi.org/10.1002/em.21762" target="_blank" title="It opens in new window">CrossRef
16. Wada, T, Kunisaki, C, Hasegawa, S, Takagawa, R, Momiyama, M, Kosaka, T, Makino, H, Ono, HA, Oshima, T, Akiyama, H, Endo, I (2013) Factors Predictive of Recurrence after Surgery for Gastric Cancer Followed by Adjuvant S-1 Chemotherapy[J]. Anticancer Res 33: pp. 1747-1751
17. Zayakin, P, Anc膩ns, G, Sili艈a, K, Meistere, I, Kalni艈a, Z, Andrejeva, D, Endzeli艈拧, E, Ivanova, L, Pismennaja, A, Ruskule, A, Doni艈a, S, Wex, T, Malfertheiner, P, Leja, M, Lin膿, A (2013) Tumor鈥恆ssociated autoantibody signature for the early detection of gastric cancer[J]. Int J Cancer 132: pp. 137-147 nal" href="http://dx.doi.org/10.1002/ijc.27667" target="_blank" title="It opens in new window">CrossRef
18. Ushijima, T, Asada, K (2010) Aberrant DNA methylation in contrast with mutations[J]. Cancer Sci 101: pp. 300-305 nal" href="http://dx.doi.org/10.1111/j.1349-7006.2009.01434.x" target="_blank" title="It opens in new window">CrossRef
19. Tsai, KW, Wu, CW, Hu, LY, Li, SC, Liao, YL, Lai, CH, Kao, HW, Fang, WL, Huang, KH, Chan, WC, Lin, WC (2011) Epigenetic regulation of miR鈥?4b and miR鈥?29 expression in gastric cancer[J]. Int J Cancer 129: pp. 2600-2610 nal" href="http://dx.doi.org/10.1002/ijc.25919" target="_blank" title="It opens in new window">CrossRef
20. Calcagno, DQ, Gigek, CO, Chen, ES, Burbano, RR, Smith, MDAC (2013) DNA and histone methylation in gastric carcinogenesis[J]. World J Gastroenterol 19: pp. 1182 nal" href="http://dx.doi.org/10.3748/wjg.v19.i8.1182" target="_blank" title="It opens in new window">CrossRef
21. Yao, D, Shi, J, Shi, B, Wang, N, Liu, W, Zhang, G, Ji, M, Xu, L, He, N, Hou, P (2012) Quantitative assessment of gene methylation and their impact on clinical outcome in gastric cancer[J]. Clin Chim Acta 413: pp. 787-794 nal" href="http://dx.doi.org/10.1016/j.cca.2012.01.013" target="_blank" title="It opens in new window">CrossRef
22. Waldmann, T, Schneider, R (2013) Targeting histone modifications鈥攅pigenetics in cancer[J]. Curr Opin Cell Biol 25: pp. 184-189 nal" href="http://dx.doi.org/10.1016/j.ceb.2013.01.001" target="_blank" title="It opens in new window">CrossRef
23. Santini, V, Kantarjian, HM, Issa, JP (2001) Changes in DNA methylation in neoplasia: pathophysiology and therapeutic implications[J]. Ann Intern Med 134: pp. 573-586 nal" href="http://dx.doi.org/10.7326/0003-4819-134-7-200104030-00011" target="_blank" title="It opens in new window">CrossRef
24. Sarkar, S, Abujamra, AL, Loew, JE, Forman, LW, Perrine, SP, Faller, DV (2011) Histone deacetylase inhibitors reverse CpG methylation by regulating DNMT1 through ERK signaling[J]. Anticancer Res 31: pp. 2723-2732
25. Santos, FPS, Kantarjian, H, Garcia-Manero, G, Issa, JP, Ravandi, F (2010) Decitabine in the treatment of myelodysplastic syndromes[J]. Expert Rev Anticancer Ther 10: pp. 9-22 nal" href="http://dx.doi.org/10.1586/era.09.164" target="_blank" title="It opens in new window">CrossRef
26. Liang, W, Xia, H, Li, J, Zhao, C, Robert, (2012) 5-Aza-2鈥?deoxycytidine increases the sensitivity of human bone marrow mesenchymal stem cells to chemotherapeutic agents by demethylation of p73[J]. J Pediatr Hematol Oncol 34: pp. 108-115 nal" href="http://dx.doi.org/10.1097/MPH.0b013e31823e0a87" target="_blank" title="It opens in new window">CrossRef
27. Tophkhane, C, Yang, SH, Jiang, Y, Ma, Z, Subramaniam, D, Anant, S, Yogosawa, S, Sakai, T, Liu, WG, Edgerton, S, Thor, A, Yang, X (2012) p53 inactivation upregulates p73 expression through E2F-1 mediated transcription[J]. PLoS One 7: pp. e43564 nal" href="http://dx.doi.org/10.1371/journal.pone.0043564" target="_blank" title="It opens in new window">CrossRef
28. Stavnes, HT, Holth, A, Don, T, K忙rn, J, Vaksman, O, Reich, R, Trope, CG, Davidson, B (2013) HOXB8 expression in ovarian serous carcinoma effusions is associated with shorter survival[J]. Gynecol Oncol 129: pp. 358-363 nal" href="http://dx.doi.org/10.1016/j.ygyno.2013.02.021" target="_blank" title="It opens in new window">CrossRef
29. Sorge, S, Ha, N, Polychronidou, M, Friedrich, J, Bezdan, D, Kaspar, P, Schaefer, MH, Ossowski, S, Henz, SR, Mundorf, J, R盲tzer, J, Papagiannouli, F, Lohmann, I (2012) The cis-regulatory code of Hox function in Drosophila[J]. EMBO J 31: pp. 3323-3333 nal" href="http://dx.doi.org/10.1038/emboj.2012.179" target="_blank" title="It opens in new window">CrossRef
30. Ahanger, SH, Srinivasan, A, Vasanthi, D, Shouche, YS, Mishra, RK (2013) Conserved boundary elements from the Hox complex of mosquito, Anopheles gambiae[J]. Nucleic Acids Res 41: pp. 804-816 nal" href="http://dx.doi.org/10.1093/nar/gks1178" target="_blank" title="It opens in new window">CrossRef
31. Javed, S, Langley, SEM (2013) The importance of HOX genes in normal prostate gland formation, prostate cancer development and its early detection[J]. BJU Int 8: pp. e12269
32. McGrath, SE, Michael, A, Pandha, H, Morgan, R (2013) Engrailed homeobox transcription factors as potential markers and targets in cancer[J]. FEBS Lett 587: pp. 549-554 nal" href="http://dx.doi.org/10.1016/j.febslet.2013.01.054" target="_blank" title="It opens in new window">CrossRef
33. Fiegl, H, Windbichler, G, Mueller-Holzner, E, Goebel, G, Lechner, M, Jacobs, IJ, Widschwendter, M (2008) HOXA11 DNA methylation鈥攁 novel prognostic biomarker in ovarian cancer[J]. Int J Cancer 123: pp. 725-729 nal" href="http://dx.doi.org/10.1002/ijc.23563" target="_blank" title="It opens in new window">CrossRef
34. Miller, C, Sassoon, DA (1998) Wnt-7a maintains appropriate uterine patterning during the development of the mouse female reproductive tract. Development 125: pp. 3201-3211 - 刊物主题：Cancer Research; Cell Biology;
- 出版者：BioMed Central
- ISSN：1475-2867
文摘
Background Aberrant DNA methylation is an acquired epigenetic alteration that serves as an alternative to genetic defects in the inactivation of tumor suppressor genes and other genes in diverse human cancers. Gastric carcinoma is one of the tumors with a high frequency of aberrant methylation in promoter region. Hence we investigated the promoter methylation status and expression level of HOXA11 gene which may involve in GC development. Methods Thirty-two surgical excised gastric cancer specimens, twelve paired adjacent non-cancerous specimens and seven normal gastric mucosas were examined. The methylation status and expression level of HOXA11 gene were determined by bisulfite sequencing polymerase chain reaction (BSP), real-time polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) respectively. HOXA11 expression was knocked-down with siRNA to mimic HOXA11 gene hypermethylation and ability of cell proliferation and migration was determinate. In addition, we analyzed and correlated the findings with clinicopathological features. Results The methylation level of HOXA11 gene in gastric cancer tissues and adjacent non-cancerous tissues were higher than those in normal gastric mucosa (P mRNA and protein decreased in normal gastric mucosa, peri-cancer tissue and GC (P siRNA in BGC-823 cells enhanced cell proliferation compared with control, but no significant different was observed in migration ability. Conclusion Hypermethylation and decreased expression of HOXA11 gene may be involved in the carcinogenesis and development of GC and may provide useful information for the prediction of the malignant behaviors of GC. And the expression of HOXA11 is impaired by DNA methylation. However, repression of HOXA11 expression promoted BGC-823 cell proliferation.