A microfluidic system for the study of the response of endothelial cells under pressure
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- 作者：Lei Li (1)
Yang Yang (1)
Xuetao Shi (2)
Hongkai Wu (2) (3)
Hongda Chen (4)
Jing Liu (1) - 关键词：Microfluidic system ; Endothelial cells ; Pressure ; Shear stress
- 刊名：Microfluidics and Nanofluidics
- 出版年：2014
- 出版时间：June 2014
- 年：2014
- 卷：16
- 期：6
- 页码：1089-1096
- 全文大小：1,009 KB
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Yang Yang (1)
Xuetao Shi (2)
Hongkai Wu (2) (3)
Hongda Chen (4)
Jing Liu (1)
1. Key Laboratory of Cryogenics & Beijing Key Laboratory of Cryo-Biomedical Engineering, Technical Institute of Physics and Chemistry (TIPC), Chinese Academy of Sciences (CAS), Beijing, 100190, China
2. WPI Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan
3. Department of Chemistry, Hongkong University of Science and Technology, Hongkong, China
4. State Key Laboratory of Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China - ISSN：1613-4990
文摘
Hydrostatic pressure can affect the structure and function of endothelial cells (ECs). A microfluidic system was built to study how ECs respond to applied pressure. The system included a syringe pump, a PDMS-glass microfluidic chip, and a digital manometer for pressure monitoring. The manometer was connected with the chip in two ways (one was before the inlet and the other after the outlet of the microchannel). The static control and flowing control systems were also set up. Human umbilical vein endothelial cells (HUVECs)?were cultured in the 4?cm?×?2?mm?×?100?μm channel. Pressure of 12?±?0.5 or 18?±?0.5?kPa was applied on the cells for 8?h. The F-actin cytoskeleton and the nuclei of the cells were stained for examination and endothelin-1 (ET-1) released from the cells in the channel was assayed by ELISA. The results showed that the cell area and ET-1 concentration increased with the pressure and a higher pressure caused more damages to the cells. This microfluidic system provides a convenient and cost-effective platform for the studies of cell response to pressure.