Screening and Identification of a Cutinase-Producing Rhodotorula mucilaginosa and Properties of the Cutinase

详细信息    查看全文

• 作者：Xiao-Ning Zhang (1) (2)
  Qin-Qin Ran (1) (2)
  Xuejun Zhang (1) (2)
  
  1. Guizhou Province Key Laboratory of Fermentation Engineering & Biological Pharmacy ; Guizhou University ; Guiyang ; 550025 ; China
  2. School of Brewing and Food Engineering ; Guizhou University ; Guiyang ; 550025 ; China
  
• 关键词：Rhodotorula mucilaginosa ; Screen ; Identification ; Cutinase ; Eucommia ulmoides
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：175
• 期：2
• 页码：1221-1233
• 全文大小：799 KB
• 参考文献：1. Lu, Z.-K., Xie, B.-X., & Du, H.-Y. (2004). / Journal of Fujian College of Forestry, 24(4), 353鈥?56.
  2. Gong, B. (2008). / Master鈥檚 thesis, Guizhou University.
  3. Gong, G.-Z., Gong, B.-H., Zhang, X.-J., Lan, X.-Y., Wang, B., & Nie, W.-Y. (2010). / Journal of Southwest University (Natural Science Edition), 07, 167鈥?72.
  4. Song, L., Zhang, X.-J., Dong, D.-P., & Wang, Q.-H. (2006). / Guizhou Chemical Industry, 04, 4鈥?.
  5. Zhang, X., Cheng, C., Zhang, M., Lan, X., Wang, Q., & Han, S. (2008). / Journal of Agricultural and Food Chemistry, 56(19), 8936鈥?943. CrossRef
  6. Murphy, C. A., Cameron, J. A., Huang, S. J., et al. (1996). / Applied and environmental microbiology, 62(2), 456鈥?60.
  7. Egmond, M. R., & de Vlieg, J. (2000). / Biochimie, 82, 1015鈥?021. CrossRef
  8. Zhang, S., & Chen, J. (2006). / Chemical Industry And Engineering Progress, 25(5), 533鈥?37.
  9. Holmquist, M. (2000). / Curr Protein Pept Sci, 1(2), 209鈥?35. CrossRef
  10. Kolattukudy, P. E., Purdy, R. E., Maiti, I. B. (1984). Cutinases from fungi and pollen. In Borg str枚m B., Brockman H. (Eds.), / Lipases, Elsevier, Amsterdam, 66, 71鈥?04.
  11. Daviesa, K. A., De Loronoa, Fosterb, S. J., Lia, D., Johnstonea, K., & Ashbya, A. M. (2000). / Physic1. Mo1. Plant Pathol, 57, 63鈥?5. CrossRef
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

Eucommia leaf contains large amounts of natural active products. In extracting the substances, the most important is the removal of the cuticle layer on the leaves and the cell wall in the leaves of Eucommia ulmoides. But the removal of the cuticle layer is a technical difficulty now. Cutinase (EC3.1.1.74) is a multifunctional enzyme with a common alpha/beta fold structure belonging to hydroplane that can make a substantial degradation of horny fatty acids. So this study isolated bacteria capable of producing cutinase from the lesion of Eucommia leaves and identified the bacteria. The identification using PCR-RFLP method confirmed that the strain belongs to Rhodotorula mucilaginosa. The fermentation conditions of the strain-producing cutinase were optimized in this study. The finding of cutinase-producing R. mucilaginosa is significant because the yeast is more secure than plant pathogens, being suitable for mass production.