Boosting immune response with the invariant chain segments via association with non-peptide binding region of major histocompatibility complex class II molecules

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• 作者：Fangfang Chen (1)
  Fantao Meng (1)
  Ling Pan (1)
  Fazhi Xu (1)
  Xuelan Liu (1)
  Weiyi Yu (1)
  
• 关键词：Li ; segments ; Epitope ; Hybrid ; MHC II ; Antibody ; Membrane co ; localization
• 刊名：BMC Immunology
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：13
• 期：1
• 全文大小：742KB
• 参考文献：1. Strubin M, Berte C, Mach B: Alternative splicing and alternative initiation of translation explain the four forms of the Ia antigen-associated invariant chain. / EMBO J 1986, 5:3483-488.
  2. Roche PA, Cresswell P: Invariant chain association with HLA-DR molecules inhibits immunogenic peptide binding. / Nature 1990, 345:615-18. CrossRef
  3. Lindner R: Transient surface delivery of invariant chain-MHC II complexes via endosomes: a quantitative study. / Traffic 2002, 3:133-46. CrossRef
  4. Cella M, Sallusto F, Lanzavecchia A: Origin, maturation and antigen presenting function of dendritic cells. / Curr Opin Immunol 1997, 9:10-6. CrossRef
  5. Saudrais C, Spehner D, de la Salle H, Bohbot A, Cazenave JP, Goud B, Hanau D, Salamero J: Intracellular pathway for the generation of functional MHC class II peptide complexes in immature human dendritic cells. / J Immunol 1998, 160:2597-607.
  6. Dugast M, Toussaint H, Dousset C, Benaroch P: AP2 clathrin adaptor complex, but not AP1, controls the access of the major histocompatibility complex (MHC) class II to endosomes. / J Biol Chem 2005, 280:19656-9664. CrossRef
  7. McCormick PJ, Martina JA, Bonifacino JS: Involvement of clathrin and AP-2 in the trafficking of MHC class II molecules to antigen-processing compartments. / Proc Natl Acad Sci USA 2005, 102:7910-915. CrossRef
  8. Schutze MP, Peterson PA, Jackson MR: An N-terminal double-arginine motif maintains type II membrane proteins in the endoplasmic reticulum. / EMBO J 1994, 13:1696-705.
  9. Stumptner P, Benaroch P: Interaction of MHC class II molecules with the invariant chain: role of the invariant chain (81-0) region. / EMBO J 1997, 16:5807-818. CrossRef
  10. Romagnoli P, Germain RN: The CLIP region of invariant chain plays a critical role in regulating major histocompatibility complex class II folding, transport, and peptide occupancy. / J Exp Med 1994, 180:1107-113. CrossRef
  11. Hsing LC, Rudensky AY: The lysosomal cysteine proteases in MHC class II antigen presentation. / Immunol Rev 2005, 207:229-41. CrossRef
  12. Busch R, Rinderknecht CH, Roh S, Lee AW, Harding JJ, Burster T, Hornell TM, Mellins ED: Achieving stability through editing and chaperoning: regulation of MHC class II peptide binding and expression. / Immunol Rev 2005, 207:242-60. CrossRef
  13. Benaroch P, Yilla M, Raposo G, Ito K, Miwa K, Geuze HJ, Ploegh HL: How MHC class II molecules reach the endocytic pathway. / EMBO J 1995, 14:37-9.
  14. Koch N, Lauer W, Habicht J, Dobberstein B: Primary structure of thegene for the murineIa antigen-associated invariant chains(Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin. / EMBO J 1987, 6:1677-683.
  15. Rudensky AY, Maric M, Eastman S, Shoemaker L, DeRoos PC, Blum JS: Intracellular assembly and transport of endogenous peptide-MHC class II complexes. / Immunity 1994, 1:585-94. CrossRef
  16. Vogt AB, Stern LJ, Amshoff C, Dobberstein B, Hammerling BJ, Kropshofer H: Interference of distinct invariant chain regions with superantigen contact area and antigenic peptide binding groove of HLA-DR. / J Immunol 1995, 155:4757-765.
  17. Ashman JB, Miller JJ: A role for the transmembrane domain in the trimerization of the MHC class II-associated invariant chain. / Immunol 1999, 163:2704-712.
  18. Frauwirth K, Shastri N: Mutation of the invariant chain transmembrane region inhibits II degradation, prolongs association with MHC class II, and selectively disrupts antigen presentation. / Cell Immunol 2001, 209:97-08. CrossRef
  19. Dixon AM, Stanley BJ, Matthews EE, Dawson JP, Engelman DM: Invariant chain transmembrane domain trimerization: a step in MHC class II assembly. / Biochemistry 2006, 45:5228-234. CrossRef
  20. Jasanoff A, Wagner G, Wiley DC: Structure of a trimeric domain of the MHC class II-associated chaperonin and targeting protein Ii. / EMBO J 1998, 17:6812-818. CrossRef
  21. Kallinteris NL, Wu S, Lu X, Humphreys RE, von Hofe E, Xu M: Enhanced CD4+ T-cell response in DR4-transgenic mice to a hybrid peptide linking the Ii-Key segment of the invariant chain to the melanoma gp100(48-8) MHC class II epitope. / J Immunother 2005, 28:352-58. CrossRef
  22. Zinckgraf JW, Sposato M, Zielinski V, Powell D, Treanor JJ, von Hofe E: Identification of HLA class II H5N1 hemagglutinin epitopes following subvirion influenza A (H5N1) vaccination. / Vaccine 2009, 27:5393-401. CrossRef
  23. Xu M, Lu X, Sposato M, Zinckgraf JW, Wu S, von Hofe E: Ii-Key/HPV16 E7 hybrid peptide immunotherapy for HPV16+ cancers. / Vaccine 2009, 27:4641-647. CrossRef
  24. Nagata T, Aoshi T, Suzuki M, Uchijima M, Kim YH, Yang Z, Koide Y: Induction of protective immunity to Listeria monocytogenes by immunization with plasmid DNA expressing a helper T-cell epitope that replaces the class II-associated invariant chain peptide of the invariant chain. / Infect Immun 2002, 70:2676-680. CrossRef
  25. Gao M, Wang HP, Wang YN, Zhou Y, Wang QL: HCV-NS3 Th1 minigene vaccine based on invariant chain CLIP genetic substitution enhances CD4(+) Th1 cell responses in vivo. / Vaccine 2006, 24:5491-. CrossRef
  26. Adams S, Humphreys RE: Invariant chain peptides enhancing or inhibiting the presentation of antigenic peptides by major histocompatibility complex class II molecules. / Eur J Immun 1995, 25:1693-702. CrossRef
  27. Xu M, Lis J, Gulfo JV: MHC class II allosteric site drugs: New immunotherapeutics for malignant, infectious and autoimmune disease. / Scan J Immunol 2001, 54:39-4. CrossRef
  28. Sotiriadou NN, Kallinteris NL, Gritzapis AD, Voutsas IF, Papamichail M, von Hofe E, Humphreys RE, Pavlis T, Perez SA, Baxevanis CN: Ii-Key/HER-2/neu(776-90) hybrid peptides induce more effective immunological responses over the native peptide in lymphocyte cultures from patients with HER-2/neu-?tumors. / Cancer Immunol Immunother 2007, 56:601-13. CrossRef
  29. Humphreys RE, Adams S, Koldzic G: Increasing the potentcy of MHC class II-presented epitopes by linking to Ii-Key peptide. / Vaccine 2000, 18:2693-697. CrossRef
  30. Gillogly ME, Kallinteris NL, Xu M, Gulfo JV, Humphreys RE, Murray JL: Ii-Key/HER-2/neu MHC class-II antigenic epitope vaccine peptide for breast cancer. / Cancer Immunol Immunother 2004, 53:490-96. CrossRef
  31. Rhyner C, Kündig T, Akdis CA, Crameri R: Targeting the MHC II presentation pathway in allergy vaccine development. / Biochem Soc Trans 2007, 35:833-34. CrossRef
  32. Gjertsson I, Laurie KL, Devitt J, Howe SJ, Thrasher AJ, Holmdahl R, Gustafsson K: Tolerance induction using lentiviral gene delivery delays onset and severity of collagen II arthritis. / Mol Ther 2009, 17:632-40. CrossRef
  33. Dong L, Yu W, Xu F, Liu G: Cloning and identifying of mouse invariant chain gene and its identification in prokaryotic cells. / J Anhui Agri Sci 2007, 35:7856-857. Chines
  34. Xu F, Wu S, Yu W: Establishment of P815 cell line stably expressing Newcastle disease virus-F gene (NDV-F). / J Agri Biotech 2009, 17:567-70. Chines
  35. Sadegh-Nasseri S, Germain RN: A role for peptide in determining MHC class II structure. / Nature 1991, 353:167-70. CrossRef
  36. Sadegh-Nasseri S, Stern LJ, Wiley DC, Germain RN: MHC class II function preserved by lowaffinity peptide interactions preceding stable binding. / Nature 1994, 370:647-50. CrossRef
  37. Sato AK, Zarutskie JA, Rushe MM, Lomakin A, Natarajan SK, Adegh-Nasseri SS: Determinants of the peptide-induced conformational change in the human class II major histocompatibility complex protein HLA-DR1. / J Biol Chem 2000, 275:2165-173. CrossRef
  38. Carven GJ, Stern LJ: Probing the ligand-induced conformational change in HLA-DR1 by selective chemical modification and mass spectrometric mapping. / Biochemistry 2005, 44:13625-3637. CrossRef
  39. Dornmair K, Rothenhausler B, McConnell HM: Structural intermediates in the reactions of antigenic peptides with MHC molecules. / Cold Spring Harb Symp Quant Biol 1989, 54:409-16. CrossRef
  40. Witt SN, McConnell HM: Formation and dissociation of short-lived class II MHC-peptide complexes. / Biochemistry 1994, 33:1861-868. CrossRef
  41. Schmitt L, Boniface JJ, Davis MM, McConnell HM: Conformational isomers of a class II MHCpeptide complex in solution. / J Mol Biol 1999, 286:207-18. CrossRef
  42. Natarajan SK, Assadi M, Sadegh-Nasser S: Stable peptide binding to MHC class II molecule is rapid and is determined by a receptive conformation shaped by prior association with low affinity peptides. / J Immunol 1999, 162:4030-036.
  43. Rabinowitz JD, Vrljic M, Kasson PM, Liang MN, Busch R, Boniface JJ: Formation of a highly peptide-receptive state of class II MHC. / Immunity 1998, 9:699-09. CrossRef
  44. Germain RN, Hendrix LR: MHC class II structure, occupancy and surface expression determined by post-endoplasmic reticulum antigen binding. / Nature 1991, 353:134-39. CrossRef
  45. Natarajan SK, Assadi M, Sadegh-Nasseri S: Stable peptide binding to MHC class II molecule is rapid and is determined by a receptive conformation shaped by prior association with low affinity peptides. / J Immunol 1999, 162:4030-036.
  46. Huby RD, Dearman RJ, Kimber I: Intracellular phosphotyrosine induction by major histocompatibility complex class II requires co-aggregation with membrane rafts. / J Biol Chem 1999, 274:22591-2596. CrossRef
  47. Karacsonyi C, Knorr R, Fulbier A, Lindner R: Association of major histocompatibility complex II with cholesterol- and sphingolipid-rich membranes precedes peptide loading. / J Biol Chem 2004, 279:34818-4826. CrossRef
  48. Anderson HA, Hiltbold EM, Roche PA: Concentration of MHC class II molecules in lipid rafts facilitates antigen presentation. / Nat Immunol 2000, 1:156-62. CrossRef
  49. Hiltbold EM, Poloso NJ, Roche PA: MHC class II-peptide complexes and APC lipid rafts accumulate at the immunological synapse. / J Immunol 2003, 170:1329-338.
  50. Pond L, Watts C: Functional early endosomes are required for maturation of major histocompatibility complex class II molecules in human B lymphoblastoid cells. / J Biol Chem 1999, 274:18049-8054. CrossRef
  51. Brachet V, Pehau-Arnaudet G, Desaymard C, Raposo G, Amigorena S: Early endosomes are required for major histocompatiblity complex class II transport to peptide-loading compartments. / Mol Biol Cell 1999, 10:2891-904.
  
• 作者单位：Fangfang Chen (1)
  Fantao Meng (1)
  Ling Pan (1)
  Fazhi Xu (1)
  Xuelan Liu (1)
  Weiyi Yu (1)
  
  1. Key Laboratory of Zoonoses of Anhui Province, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China
  
• ISSN：1471-2172

文摘

Background Based on binding of invariant chain (Ii) to major histocompatibility complex (MHC) class II molecules to form complexes, Ii-segment hybrids, Ii-key structure linking an epitope, or Ii class II-associated invariant chain peptide (CLIP) replaced with an epitope were used to increase immune response. It is currently unknown whether the Ii-segment cytosolic and transmembrane domains bind to the MHC non-peptide binding region (PBR) and consequently influence immune response. To investigate the potential role of Ii-segments in the immune response via MHC II/peptide complexes, a few hybrids containing Ii-segments and a multiepitope (F306) from Newcastle disease virus fusion protein (F) were constructed, and their binding effects on MHC II molecules and specific antibody production were compared using confocal microscopy, immunoprecipitation, western blotting and animal experiments. Results One of the Ii-segment/F306 hybrids, containing ND (Asn–Asp) outside the F306 in the Ii-key structure (Ii-key/F306/ND), neither co-localized with MHC II molecules on plasma membrane nor bound to MHC II molecules to form complexes. However, stimulation of mice with the structure produced 4-fold higher antibody titers compared with F306 alone. The two other Ii-segment/F306 hybrids, in which the transmembrane and cytosolic domains of Ii were linked to this structure (Cyt/TM/Ii-key/F306/ND), partially co-localized on plasma membrane with MHC class II molecules and weakly bound MHC II molecules to form complexes. They induced mice to produce approximately 9-fold higher antibody titers compared with F306 alone. Furthermore, an Ii/F306 hybrid (F306 substituting CLIP) co-localized well with MHC II molecules on the membrane to form complexes, although it increased antibody titer about 3-fold relative to F306 alone. Conclusions These results suggest that Ii-segments improve specific immune response by binding to the non-PBR on MHC class II molecules and enabling membrane co-localization with MHC II molecules, resulting in the formation of relatively stable MHC II/peptide complexes on the plasma membrane, and signal transduction.