Purification and Characterization of a Glucosamine-Binding Antifungal Lectin from Phaseolus vulgaris cv. Chinese Pinto Beans with Antiproliferative Activity Towards Nasopharyngeal Carcinoma Cells

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• 作者：Andrew Si Wo Ang (1)
  Randy Chi Fai Cheung (2)
  Xiuli Dan (2)
  Yau Sang Chan (2)
  Wenliang Pan (2)
  Tzi Bun Ng (2)
  
• 关键词：Phaseolus vulgaris ; Antifungal ; Antitumor ; Lectin ; Nasopharyngeal carcinomas
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：172
• 期：2
• 页码：672-686
• 全文大小：381 KB
• 作者单位：Andrew Si Wo Ang (1)
  Randy Chi Fai Cheung (2)
  Xiuli Dan (2)
  Yau Sang Chan (2)
  Wenliang Pan (2)
  Tzi Bun Ng (2)
  
  1. Faculty of Science, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China
  2. Faculty of Medicine, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China
  
• ISSN：1559-0291

文摘

A lectin has successfully been isolated from Phaseolus vulgaris cv. Chinese pinto bean using affinity chromatography, ion exchange chromatography, and gel filtration in succession, with a 15.4-fold purification. Investigation of its characteristics revealed that Chinese pinto bean lectin (CPBL) was a 58-kDa dimeric glucosamine-binding protein. Its Mg2+-dependent hemagglutinating activity was stable at pH 7- and at or below 60?°C. When the purified lectin was tested against six fungal species including Phyllosticta citriasiana, Magnaporthe grisea, Bipolans maydis, Valsa mali, Mycosphaerella arachidicola, and Setosphaeria turcica, only the mycelial growth of V. mali was reduced by 30.6?% by the lectin at 30?μM. The lectin did not exert any discernible antiproliferative effects on breast cancer MCF-7 cells, but was able to suppress proliferation of nasopharyngeal carcinoma HONE-1 cells, with an IC50sub> of 17.3?μM, as revealed by the MTT assay. Since few plant lectins demonstrate antifungal activity against V. mali, and not many others have inhibitory effects on HONE-1 cells, CPBL is a distinctive lectin which may be exploited for development into an agent against V. mali and HONE-1 cells.