Suitable extracellular oxidoreduction potential inhibit rex regulation and effect central carbon and energy metabolism in Saccharopolyspora spinosa

详细信息    查看全文

• 作者：Xiangmei Zhang (1) (2) (3)
  Chaoyou Xue (1) (2) (3)
  Fanglong Zhao (1) (2) (3)
  Dashuai Li (1) (2) (3)
  Jing Yin (1) (2) (3)
  Chuanbo Zhang (1) (2) (3)
  Qinggele Caiyin (1) (2) (3)
  Wenyu Lu (1) (2) (3)
  
  1. Department of Biological Engineering ; School of Chemical Engineering and Technology ; Tianjin University ; Tianjin ; 300072 ; PR China
  2. Key Laboratory of system bioengineering (Tianjin University) ; Ministry of Education ; Tianjin ; 300072 ; PR China
  3. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) ; Tianjin ; 300072 ; PR China
  
• 关键词：Saccharopolyspora spinosa ; Oxidative condition ; H2O2 ; Rex ; Metabolites
• 刊名：Microbial Cell Factories
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：13
• 期：1
• 全文大小：1,121 KB
• 参考文献：1. Madduri, K, Waldron, C, Matsushima, P, Broughton, MC, Crawford, K, Merlo, DJ, Baltz, RH (2001) Genes for the biosynthesis of spinosyns: applications for yield improvement in Saccharopolyspora spinosa. J Ind Microbiol Biotechnol 27: pp. 399-402 CrossRef
  2. Sparks, TC, Crouse, GD, Durst, G (2001) Natural products as insecticides: the biology, biochemistry and quantitative structure鈥揳ctivity relationships of spinosyns and spinosoids. Pest Manag Sci 57: pp. 896-905 CrossRef
  3. Williams, T, Valle, J, Vi帽uela, E (2003) Is the naturally derived insecticide Spinosad庐 compatible with insect natural enemies?. Biocontrol Sci Tech 13: pp. 459-475 CrossRef
  4. Kirst, HA (2010) The spinosyn family of insecticides: realizing the potential of natural products research. J Antibiot 63: pp. 101-111 CrossRef
  5. Sarfraz, M, Dosdall, LM, Keddie, BA (2005) Spinosad: a promising tool for integrated pest management. Outlook Pest Manage 16: pp. 78-84 CrossRef
  6. Huang, K, Xia, L, Zhang, Y, Ding, X, Zahn, JA (2009) Recent advances in the biochemistry of spinosyns. Appl Microbiol Biotechnol 82: pp. 13-23 CrossRef
  7. Pan, HX, Li, JA, He, NJ, Chen, JY, Zhou, YM, Shao, L, Chen, DJ (2011) Improvement of spinosad production by overexpression of gtt and gdh controlled by promoter PermE* in Saccharopolyspora spinosa SIPI-A2090. Biotechnol Lett 33: pp. 733-739 CrossRef
  8. Xue, C, Duan, Y, Zhao, F, Lu, W (2013) Stepwise increase of spinosad production in Saccharopolyspora spinosa by metabolic engineering. Biochem Eng J 72: pp. 90-95 CrossRef
  9. Liang, Y, Lu, W, Wen, J (2009) Improvement of Saccharopolyspora spinosa and the kinetic analysis for spinosad production. Appl Biochem Biotechnol 152: pp. 440-448 CrossRef
  10. Strobel, RJ, Nakatsukasa, WM (1993) Response surface methods for optimizing Saccharopolyspora spinosa, a novel macrolide producer. J Ind Microbiol 11: pp. 121-127 CrossRef
  11. Brekasis, D, Paget, MSB (2003) A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3 (2). EMBO J 22: pp. 4856-4865 CrossRef
  12. Green, J, Paget, MS (2004) Bacterial redox sensors. Nat Rev Microbiol 2: pp. 954-966 CrossRef
  13. Gonz谩lez-Siso, MI, Garc铆a-Leiro, A, Tarr铆o, N, Cerd谩n, ME (2009) Sugar metabolism, redox balance and oxidative stress response in the respiratory yeast Kluyveromyces lactis. Microb Cell Factories 8: pp. 46 CrossRef
  14. Liu, CG, Xue, C, Lin, YH, Bai, FW (2013) Redox potential control and applications in microaerobic and anaerobic fermentations. Biotechnol Adv 31: pp. 257-265 CrossRef
  15. Ravcheev, DA, Li, X, Latif, H, Zengler, K, Leyn, SA, Korostelev, YD, Rodionov, DA (2012) Transcriptional regulation of central carbon and energy metabolism in bacteria by redox-responsive repressor Rex. J Bacteriol 194: pp. 1145-1157 CrossRef
  16. Riondet, C, Cachon, R, Wach茅, Y, Alcaraz, G, Divi猫s, C (2000) Extracellular oxidoreduction potential modifies carbon and electron flow in Escherichia coli. J Bacteriol 182: pp. 620-626 CrossRef
  17. Kelly, DJ Metabolism, electron transport and bioenergetics of Campylobacter jejuni: implications for understanding life in the gut and survival in the environment. In: Ketley, JM, Konkel, ME eds. (2005) Campylobacter, Molecular and Cellular Biology. Horizon Bioscience, Wymondham, UK, pp. 275-292
  18. Amanullah, A, Blair, R, Nienow, AW, Thomas, CR (1999) Effects of agitation intensity on mycelial morphology and protein production in chemostat cultures of recombinant Aspergillus oryzae. Biotechnol Bioeng 62: pp. 434-446 CrossRef
  19. Carata, E, Peano, C, Tredici, SM, Ferrari, F, Tal脿, A, Corti, G, Alifano, P (2009) Phenotypes and gene expression profiles of Saccharopolyspora erythraea rifampicin-resistant (rif) mutants affected in erythromycin production. Microb Cell Fact 8: pp. 18 CrossRef
  20. Wang, E, Bauer, MC, Rogstam, A, Linse, S, Logan, DT, Von Wachenfeldt, C (2008) Structure and functional properties of the Bacillus subtilis transcriptional repressor Rex. Mol Microbiol 69: pp. 466-478 CrossRef
  21. Borodina, I, Siebring, J, Zhang, J, Smith, CP, Van Keulen, G, Dijkhuizen, L, Nielsen, J (2008) Antibiotic overproduction in Streptomyces coelicolor A3 (2) mediated by phosphofructokinase deletion. J Biol Chem 283: pp. 25186-25199 CrossRef
  22. Peng, L, Shimizu, K (2003) Global metabolic regulation analysis for Escherichia coli K12 based on protein expression by 2-dimensional electrophoresis and enzyme activity measurement. Appl Microbiol Biotechnol 61: pp. 163-178 CrossRef
  23. Gramajo, HC, Takano, E, Bibb, MJ (1993) Stationary phase production of the antibiotic actinorhodin in Streptomyces coelicolor A3 (2) is transcriptionally regulated. Mol Microbiol 7: pp. 837-845 CrossRef
  24. Staunton, J, Weissman, KJ (2001) Polyketide biosynthesis: a millennium review. Nat Prod Rep 18: pp. 380-416 CrossRef
  25. Simon, R, Priefer, U, P眉hler, A (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Nat Biotechnol 1: pp. 784-791 CrossRef
  26. Mertz, FP, Yao, RC (1990) Saccharopolyspora spinosa sp. nov. isolated from soil collected in a sugar mill rum still. Int J Syst Bacteriol 40: pp. 34-39 CrossRef
  27. Bierman, M, Logan, R, O'brien, K, Seno, ET, Nagaraja Rao, R, Schoner, BE (1992) Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116: pp. 43-49 CrossRef
  28. Matsushima, P, Broughton, MC, Turner, JR, Baltz, RH (1994) Conjugal transfer of cosmid DNA from Escherichia coli to Saccharopolyspora spinosa: effects of chromosomal insertions on macrolide A83543 production. Gene 146: pp. 39-45 CrossRef
  29. Puertas, JM, Betton, JM (2009) Engineering an efficient secretion of leech carboxypeptidase inhibitor in Escherichia coli. Microb Cell Factories 8: pp. 57 CrossRef
  30. Miller, GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: pp. 426-428 CrossRef
  31. Berr谋虂os-Rivera, SJ, Bennett, GN, San, KY (2002) The effect of increasing NADH availability on the redistribution of metabolic fluxes in Escherichia coli chemostat cultures. Metab Eng 4: pp. 230-237 CrossRef
  32. Lin, AP, McAlister-Henn, L (2002) Isocitrate binding at two functionally distinct sites in yeast NAD+-specific isocitrate dehydrogenase. J Biol Chem 277: pp. 22475-22483 CrossRef
  33. Ryu, YG, Butler, MJ, Chater, KF, Lee, KJ (2006) Engineering of primary carbohydrate metabolism for increased production of actinorhodin in Streptomyces coelicolor. Appl Environ Microbiol 72: pp. 7132-7139 CrossRef
  34. Xue, C, Zhang, X, Yu, Z, Zhao, F, Wang, M, Lu, W (2013) Up-regulated spinosad pathway coupling with the increased concentration of acetyl-CoA and malonyl-CoA contributed to the increase of spinosad in the presence of exogenous fatty acid. Biochem Eng J 81: pp. 47-53 CrossRef
  35. Ding, MZ, Cheng, JS, Xiao, WH, Qiao, B, Yuan, YJ (2009) Comparative metabolomic analysis on industrial continuous and batch ethanol fermentation processes by GC-TOF-MS. Metabolomics 5: pp. 229-238 CrossRef
  36. Demoz, A, Garras, A, Asiedu, DK, Netteland, B, Berge, RK (1995) Rapid method for the separation and detection of tissue short-chain coenzyme A esters by reversed-phase high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl 667: pp. 148-152 CrossRef
  37. Creemer, LC, Kirst, HA, Paschal, JW (1998) Conversion of spinosyn A and spinosyn D to their respective 9-and 17-pseudoaglycones and their aglycones. J Antibiot 51: pp. 795 CrossRef
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Applied Microbiology
  Environmental Engineering/Biotechnology
  
• 出版者：BioMed Central
• ISSN：1475-2859

文摘

Background Polyketides, such as spinosad, are mainly synthesized in the stationary phase of the fermentation. The synthesis of these compounds requires many primary metabolites, such as acetyl-CoA, propinyl-CoA, NADPH, and succinyl-CoA. Their synthesis is also significantly influenced by NADH/NAD+. Rex is the sensor of NADH/NAD+ redox state, whose structure is under the control of NADH/NAD+ ratio. The structure of rex controls the expression of many NADH dehydrogenases genes and cytochrome bd genes. Intracellular redox state can be influenced by adding extracellular electron acceptor H2O2. The effect of extracellular oxidoreduction potential on spinosad production has not been studied. Although extracellular oxidoreduction potential is an important environment effect in polyketides production, it has always been overlooked. Thus, it is important to study the effect of extracellular oxidoreduction potential on Saccharopolyspora spinosa growth and spinosad production. Results During stationary phase, S. spinosa was cultured under oxidative (H2O2) and reductive (dithiothreitol) conditions. The results show that the yield of spinosad and pseudoaglycone increased 3.11 fold under oxidative condition. As H2O2 can be served as extracellular electron acceptor, the ratios of NADH/NAD+ were measured. We found that the ratio of NADH/NAD+ under oxidative condition was much lower than that in the control group. The expression of cytA and cytB in the rex mutant indicated that the expression of these two genes was controlled by rex, and it was not activated under oxidative condition. Enzyme activities of PFK, ICDH, and G6PDH and metabolites results indicated that more metabolic flux flow through spinosad synthesis. Conclusion The regulation function of rex was inhibited by adding extracellular electron acceptor-H2O2 in the stationary phase. Under this condition, many NADH dehydrogenases which were used to balance NADH/NAD+ by converting useful metabolites to useless metabolites and unefficient terminal oxidases (cytochrome bd) were not expressed. So lots of metabolites were not waste to balance. As a result, un-wasted metabolites related to spinosad and PSA synthesis resulted in a high production of spinosad and PSA under oxidative condition.