Synthesis and Characterization of a Molecularly Imprinted Silica Gel Sorbent for the On-Line Determination of Trace Sudan I in Chilli Powder through High-Performance Liquid Chromatography

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• 作者：Shuo Wang ; Zhixiang Xu ; Guozhen Fang ; Zhenjuan Duan ; Yan Zhang ; Shuang Chen
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2007
• 出版时间：May 16, 2007
• 年：2007
• 卷：55
• 期：10
• 页码：3869 - 3876
• 全文大小：189K
• 年卷期：v.55,no.10(May 16, 2007)
• ISSN：1520-5118

文摘

A highly selective imprinted polymer was synthesized by a surface molecular imprinting technique incombination with a sol-gel process. The imprinted polymer was evaluated by FT-IR and static andkinetic adsorption experiments. The results showed that the imprinted sorbent exhibited goodrecognition and selective ability, offered a faster kinetics for the adsorption and desorption of SudanI than the non-imprinted sorbent, a saturated binding capacity (Q_max) that reached 33.47 mg g^-1.The prepared sorbent was applied for the determination of trace Sudan I through on-line solid-phasecoupled with high-performance liquid chromatography (SPE-HPLC). With a loading flow rate of 1.5mL min^-1 for sampling 50 mL, an enrichment factor of 1266 was achieved. The detection limit (S/N= 3) was 1.2 ng L^-1, and the peak area precision (RSD) for five replicate detections of 0.01 g L^-1Sudan I was 3.66%. The Sudan I in the chilli powder from the local market was determined at threelevels (0.25, 0.5, and 1.0 ng g^-1) with recoveries ranging from 80.31 to 94.02%.