T
he pentapeptide Cys-Ala-Leu-Asn-Asn (CALNN) has been proved to be a powerful tool to stabilize t
he AuNPs. T
hese CALNN-capped AuNPs have been used to develop various bioanalysis platforms. In this paper, t
he CALNN-capped AuNPs are proved to be a robust tool for aggregation-based colorimetric immunoassays as well. A colorimetric immunoassay strategy based upon t
he antibody-induced assembly of functionalized AuNPs for Abscisic Acid glucose ester (ABA-GE) determination has been developed. T
he ABA-functionalized AuNPs aggregate in t
he presence of specific antibody, accompanied by a color change of t
he solution. T
he color change is competitively inhibited by ABA-GE. T
he interparticle distance in aggregates is small due to t
he thin peptide layer on t
he AuNPs surface, and it is determined by t
he 鈥淵鈥?shape antibody linker as well. As a result of that, an obvious color change in t
he immunoassays is observed. Under t
he optimized conditions, a linear response range from 5 nM to 10 渭M for ABA-GE determination is obtained, and t
he limit of detection (LOD) is evaluated to be 2.2 nM. This method is simple, homogeneous, and has potential for visual detection of ABA-GE.
Keywords:
peptide; CALNN; gold nanoparticle; colorimetric immunoassay; plant hormone; conjugated abscisic acid