文摘
An aldehyde oxime O-methyl ether, phenylacetaldehyde O-methyloxime, was detected using solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) in theheadspace above grapefruit leaves infected with Xanthomonas axonopodis pv. citri, the causal agentof citrus bacterial canker disease (CBCD). This disease is a major phytosanitary concern, and aneradication campaign against it is currently underway in Florida. Phenylacetaldehyde O-methyloximehas been reported to be produced by other plants and fragrant flowers, but it was not observed inthe headspace above uninfected grapefruit leaves, the pathogenic bacterium X. axonopodis pv. citriitself, or grapefruit leaves infected with another closely related bacterial pathogen, X. axonopodis pv.citrumelo, which causes citrus bacterial spot, a disease of no phytosanitary significance. It was alsonot detected from CBCD infected fruits, including orange, lemon, grapefruit, and lime. We concludethat phenylacetaldehyde O-methyloxime may potentially be used to identify CBCD infestations.However, more intensive studies will be required to fully evaluate the potential of phenylacetaldehydeO-methyloxime as a diagnostic compound for CBCD. Using SPME and GC-MS to measurephenylacetaldehyde O-methyloxime may provide an easy and feasible tool to complement currentmethods used to detect X. axonopodis pv. citri in environmental samples.Keywords: Phenylacetaldehyde O-methyloxime; citrus bacterial canker disease; Xanthomonasaxonopodis pv. citri; Xanthomonas axonopodis pv. citrumelo; solid-phase microextraction