Differential Proteomic Analysis of a Polymicrobial Biofilm

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• 作者：Zamirah Zainal-Abidin ; Paul D. Veith ; Stuart G. Dashper ; Ying Zhu ; Deanne V. Catmull ; Yu-Yen Chen ; Deasy C. Heryanto ; Dina Chen ; James S. Pyke ; Kheng Tan ; Helen L. Mitchell ; Eric C. Reynolds
• 刊名：Journal of Proteome Research
• 出版年：2012
• 出版时间：September 7, 2012
• 年：2012
• 卷：11
• 期：9
• 页码：4449-4464
• 全文大小：710K
• 年卷期：v.11,no.9(September 7, 2012)
• ISSN：1535-3907

文摘

Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia exist in a polymicrobial biofilm associated with chronic periodontitis. The aim of this study was to culture these three species as a polymicrobial biofilm and to determine proteins important for bacterial interactions. In a flow cell all three species attached and grew as a biofilm; however, after 90 h of culture P. gingivalis and T. denticola were closely associated and dominated the polymicrobial biofilm. For comparison, planktonic cultures of P. gingivalis and T. denticola were grown separately in continuous culture. Whole cell lysates were subjected to SDS-PAGE, followed by in-gel proteolytic H₂¹⁶O/H₂¹⁸O labeling. From two replicates, 135 and 174 P. gingivalis proteins and 134 and 194 T. denticola proteins were quantified by LC鈥揗ALDI TOF/TOF MS. The results suggest a change of strategy in iron acquisition by P. gingivalis due to large increases in the abundance of HusA and HusB in the polymicrobial biofilm while HmuY and other iron/haem transport systems decreased. Significant changes in the abundance of peptidases and enzymes involved in glutamate and glycine catabolism suggest syntrophy. These data indicate an intimate association between P. gingivalis and T. denticola in a biofilm that may play a role in disease pathogenesis.

Keywords:

bacterial community; Porphyromonas gingivalis; Treponema denticola; Tannerella forsythia; ¹⁸O; quantitative proteomics; LC鈭扢ALDI-TOF/TOF; iron; glycine; protein secretion; chronic periodontitis