Effect of Medium Composition on Production of Striga hermonthica (Del.) Benth Germination Stimulant(s) by Menispermum dauricum (DC.) Root Cultures

详细信息    查看全文

• 作者：Yongqing Ma ; Abdel Gabar T. Babiker ; Yukihiro Sugimoto ; and Shinobu Inanaga
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1998
• 出版时间：April 1998
• 年：1998
• 卷：46
• 期：4
• 页码：1587 - 1592
• 全文大小：428K
• 年卷期：v.46,no.4(April 1998)
• ISSN：1520-5118

文摘

Germination of Striga hermonthica (Del.) Benth, a noxiousparasitic weed on cereals, requires anexogenous stimulant produced by the roots of host and some nonhostplant species. Root culturesof Menispermum dauricum (DC.), a nonhostbroad-leaved herbaceous plant, produced a group ofsubstances that induce the parasite germination. This paperreports the establishment of a high-stimulant-producing M. dauricum root culture by manipulationof culture composition. A modifiedB5 medium (MB5) containing 35.7 mM nitrogen at aNO₃^-/NH₄⁺ ratio of1:42, 0.1 mM Fe²⁺, 1.0mM Ca²⁺, 0.55 mM inorganic phosphorus, 0.28 mM inositol,4.1 mages/entities/mgr.gif">M nicotinic acid, 3.7 mages/entities/mgr.gif">M pyridoxinehydrochloride, 14.8 mages/entities/mgr.gif">M thiamin hydrochloride, 1 mages/entities/mgr.gif">M1-naphthaleneacetic acid (NAA), and 4% sucrosesustained root growth for a longer period and increased root biomass by>30% and stimulantproduction by 5-fold, in comparison to the standard B5 mediumsupplemented with 3% sucrose and1 mages/entities/mgr.gif">M NAA.Keywords: Root culture; germination stimulant; Strigahermonthica