Glutathione-Triggered 鈥淥ff鈥揙n鈥?Release of Anticancer Drugs from Dendrimer-Encapsulated Gold Nanoparticles

详细信息    查看全文

• 作者：Xinyu Wang ; Xiaopan Cai ; Jingjing Hu ; Naimin Shao ; Fei Wang ; Qiang Zhang ; Jianru Xiao ; Yiyun Cheng
• 刊名：The Journal of the American Chemical Society
• 出版年：2013
• 出版时间：July 3, 2013
• 年：2013
• 卷：135
• 期：26
• 页码：9805-9810
• 全文大小：477K
• 年卷期：v.135,no.26(July 3, 2013)
• ISSN：1520-5126

文摘

Polymeric nanoparticles that can stably load anticancer drugs and release them in response to a specific trigger such as glutathione are of great interest in cancer therapy. In the present study, dendrimer-encapsulated gold nanoparticles (DEGNPs) were synthesized and used as carriers of thiolated anticancer drugs. Thiol-containing drugs such as captopril and 6-mercaptopurine loaded within DEGNPs showed an 鈥淥ff鈥揙n鈥?release behavior in the presence of thiol-reducing agents such as glutathione and dithiothreitol. Thiolated doxorubicin and cisplatin, loaded within the nanoparticle, showed much reduced cytotoxicity as compared to the free anticancer compounds. The toxicity of drug-loaded DEGNPs can be enhanced by improving the intracellular glutathione. Glutathione-triggered release of thiolated doxorubicin within cancer cells is further confirmed by flow cytometry and confocal laser scan microscopy studies. In addition, DEGNPs showed excellent biocompatibility on several cell lines. This study provides a new insight into biomedical applications of dendrimers and dendrimer-encapsulated nanoparticles.