Water-Soluble Poly(纬-propargyl-l-glutamate) Containing Pendant Sulfonate Ions and Terminal Fluorophore: Aggregation-Enhanced Emission and Secondary Structure

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• 作者：Ke-Ying Shih ; Tai-Shen Hsiao ; Shiang-Lin Deng ; Jin-Long Hong
• 刊名：Macromolecules
• 出版年：2014
• 出版时间：June 24, 2014
• 年：2014
• 卷：47
• 期：12
• 页码：4037-4047
• 全文大小：696K
• ISSN：1520-5835

文摘

Tetraphenylthiophene (TP) with aggregation-enhanced emission (AEE) property was used as terminal fluorophore of the water-soluble poly(纬-propargyl-l-glutamate) (PPLG)-based polymers of TP-iPPLGs to probe the relationship between the secondary structure (伪-helix) of polypeptides and the AEE-related emission behavior. Intermolecular aggregation of the terminal TP unit in TP-iPPLG is sterically blocked by the large 伪-helical PPLG chains, leading to the weak AEE-related fluorescence in water. In contrast, the intermolecular approach between the terminal TP units of TP-iPPLG is accessible if TP was connected by peptide chin in random coil structure. Therefore, the helix-to-coil transition induced in the alkaline aqueous solution successfully enhances the emission intensity of the TP-iPPLG solution. The pH-induced conformation change in relationship to the AEE-related emission behavior is therefore evaluated in this study.