Electrochemistry Investigation on Protein Protection by Alkanethiol Self-Assembled Monolayers against Urea Impact
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- 作者：Yan Guo ; Jianwei Zhao ; Xing Yin ; Xiaoling Gao ; Yanni Tian
- 刊名：Journal of Physical Chemistry C
- 出版年：2008
- 出版时间：April 17, 2008
- 年：2008
- 卷：112
- 期：15
- 页码：6013 - 6021
- 全文大小：219K
- 年卷期：v.112,no.15(April 17, 2008)
- ISSN：1932-7455
文摘
The present investigation reports the electrochemical measurements of azurin (Az) adsorbed on a series ofalkanethiol self-assembled monolayers (SAMs) under the influence of urea molecules. Theoretical fittingwith the Marcus model obtains the electron-transfer rate constant, ket, and the reorganization energy, 
. Whenthe underlying SAM is longer than 10 methylene units, ket shows an obvious chain-length dependence fromwhich an electron-tunneling coefficient, 
, of 1.09 per methylene is deduced. Combined with cyclicvoltammetric results, variations of both ket and imply that urea impact does not penetrate into the ion corepart of Az but instead influences the network of molecular hydrogen bonds. The mechanism of urea impactis further discussed by means of the pH dependence of the equilibrium potential.
. Whenthe underlying SAM is longer than 10 methylene units, ket shows an obvious chain-length dependence fromwhich an electron-tunneling coefficient, , of 1.09 per methylene is deduced. Combined with cyclicvoltammetric results, variations of both ket and imply that urea impact does not penetrate into the ion corepart of Az but instead influences the network of molecular hydrogen bonds. The mechanism of urea impactis further discussed by means of the pH dependence of the equilibrium potential.