On-Plate Selective Enrichment and Self-Desalting of Peptides/Proteins for Direct MALDI MS Analysis

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• 作者：Zhoufang Zeng ; Yandong Wang ; Shoulei Shi ; Lifeng Wang ; Xinhua Guo ; Nan Lu
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：March 6, 2012
• 年：2012
• 卷：84
• 期：5
• 页码：2118-2123
• 全文大小：265K
• 年卷期：v.84,no.5(March 6, 2012)
• ISSN：1520-6882

文摘

In this paper, a new technique has been proposed to achieve simultaneous peptides/proteins enrichment and wash-free self-desalting on a novel sample support with a circle hydrophobic鈥揾ydrophilic鈥揾ydrophobic pattern. Upon deposition, the sample solution is first concentrated in a small area by repulsion of the hydrophobic outer layer, and then, the peptides/proteins and coexisting salt contaminants are selectively captured in different regions of the pattern through strong hydrophobic and hydrophilic attractions, respectively. As a result, the detection sensitivity is improved by 2 orders of magnitude better than the use of the traditional MALDI plate, and high-quality mass spectra are obtained even in the presence of NaCl (1 M), NH₄HCO₃ (100 mM), or urea (1 M). The practical application of this method is further demonstrated by the successful analysis of myoglobin digests with high sequence coverage, demonstrating the great potential in proteomic research.