Hydrogen Isotopic Profile in the Characterization of Sugars. Influence of the Metabolic Pathway

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• 作者：Ben-Li Zhang ; Isabelle Billault ; Xiaobao Li ; Franç ; oise Mabon ; Gé ; rald Remaud ; and Maryvonne L. Martin
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2002
• 出版时间：March 13, 2002
• 年：2002
• 卷：50
• 期：6
• 页码：1574 - 1580
• 全文大小：74K
• 年卷期：v.50,no.6(March 13, 2002)
• ISSN：1520-5118

文摘

The site-specific natural hydrogen isotope ratios of plant metabolites determined by ²H nuclearmagnetic resonance (SNIF-NMR method) can provide powerful criteria for inferring mechanistic andenvironmental effects on biosynthetic pathways. This work examines the potential of isotopic profilesfor the main constituents of carbohydrates, glucose and fructose, to distinguish different photosyntheticpathways. An appropriate analytical strategy, involving three suitable isotopic probes, has beenelaborated with a view to measuring simultaneously, in conditions devoid of isotopic perturbations,all (or nearly all) of the carbon-bound hydrogen isotope ratios. It is shown that the type of photosyntheticmetabolism, either C3 (sugar beet, orange, and grape), C4 (maize and sugar cane), or CAM(pineapple), and the physiological status of the precursor plant exert strong influences on the deuteriumdistribution in the sugar molecules. Consequently, this isotopic fingerprint may be a rich source ofinformation for the comparison of mechanisms in metabolic pathways. In addition, it can providecomplementary criteria to ethanol as a probe for the origin of sugars.Keywords: Glucose; fructose; plant metabolism; isotope ratio; deuterium; SNIF-NMR