Analysis of Trace Microcystins in Vegetables Using Solid-Phase Extraction Followed by High Performance Liquid Chromatography Triple-Quadrupole Mass Spectrometry
详细信息 查看全文
- 作者：Yan-Wen Li ; Xiao-Jing Zhan ; Lei Xiang ; Zhe-Shen Deng ; Bin-Hui Huang ; Hong-Fei Wen ; Teng-Fei Sun ; Quan-Ying Cai ; Hui Li ; Ce-Hui Mo
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2014
- 出版时间：December 10, 2014
- 年：2014
- 卷：62
- 期：49
- 页码：11831-11839
- 全文大小：414K
- ISSN：1520-5118
文摘
A selective and sensitive method for the simultaneous detection of three common and hazardous microcystins (microcystins-LR, -RR, and -YR) in various vegetables was established using solid-phase extraction followed by high performance liquid chromatography coupled with mass spectrometry. The methanol鈥搘ater proportion ratio of the extraction solvent and its acidity, as well as the efficiencies of solid-phase extraction, were evaluated to optimize a pretreatment procedure for extracting the microcystins from 10 vegetable matrices. The limits of detection and quantitation were below 7.5 渭g/kg (dw) and 25 渭g/kg (dw), respectively, in different vegetable matrices. The recoveries of the microcystins in the 10 vegetable matrices ranged from 61.3 to 117.3%, with RSDs of 0.2鈥?8.3%. The established method was used to analyze 28 field vegetable samples collected from the sides of Lake Dianchi, and microcystin-RR was found in almost all samples at concentrations of 36.4鈥?352.2 渭g/kg (dw).