Nanoparticles That Display Short Collagen-Related Peptides. Potent Stimulation of Human Platelet Aggregation by Triple Helical Motifs

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• 作者：Mabel A. Cejas ; Cailin Chen ; William A. Kinney ; Bruce E. Maryanoff
• 刊名：Bioconjugate Chemistry
• 出版年：2007
• 出版时间：July 2007
• 年：2007
• 卷：18
• 期：4
• 页码：1025 - 1027
• 全文大小：441K
• 年卷期：v.18,no.4(July 2007)
• ISSN：1520-4812

文摘

Collagen plays a key role in the activation and adhesion of blood platelets via their cell-surface receptors. Normally,collagen-related peptides (CRPs), even one as long as a 30-mer (10 Gly-Pro-Hyp (GPO) repeats), are unable toeffectively express collagen's platelet-activating behavior. We attached two short CRPs, AcHN-(Gly-Pro-Hyp)_nGly-OH with n = 5 (1) and n = 10 (2), via the C-terminus to amino-functionalized latex nanoparticles to create amultimeric display of triple helical motifs. These nanomaterials were characterized by dynamic light scatteringand environmental scanning electron microscopy. The nanoparticles bearing the 31-mer CRP sequence, 2, butnot the 16-mer sequence, 1, effectively induced the aggregation of human platelets, with a potency level approachingthat of native type I collagen. Our results highlight the importance of presenting triple helical CRP motifs ofsufficient length on a suitable scaffold in order to stimulate platelets.