Europium-Doped TiO2 Hollow Nanoshells: Two-Photon Imaging of Cell Binding

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• 作者：Sergio Sandoval ; Jian Yang ; Jesus G. Alfaro ; Alexander Liberman ; Milan Makale ; Casey E. Chiang ; Ivan K. Schuller ; Andrew C. Kummel ; William C. Trogler
• 刊名：Chemistry of Materials
• 出版年：2012
• 出版时间：November 13, 2012
• 年：2012
• 卷：24
• 期：21
• 页码：4222-4230
• 全文大小：605K
• 年卷期：v.24,no.21(November 13, 2012)
• ISSN：1520-5002

文摘

A simple scalable method to fabricate luminescent monodisperse 200 nm europium-doped hollow TiO₂ nanoshell (NS) particles is reported. Fluorophore reporter, Eu³⁺ ions are incorporated directly in the NS matrix, leaving the surface free for functionalization and the core free for payload encapsulation. Amine-functionalized polystyrene beads were used as templates, and the porous walls of europium-doped titania nanoshells were synthesized using titanium(IV) t-butoxide and europium(III) nitrate as reactants. X-ray diffraction analysis identified anatase as the predominant titania phase of the rigid nanoshell wall structure, and photoluminescence spectra showed that the Eu(III)-doped TiO₂ nanoshells exhibited a red emission at 617 nm due to an atomic f鈥揻 transition. Nanoshell interactions with HeLa cervical cancer cells in vitro were visualized using two-photon microscopy of the Eu(III) emission and studied using a luminescence ratio analysis to assess nanoshell adhesion and endocytosis.
<h4>Keywords: h4> hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=two+photon&qsSearchArea=searchText">two photon; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=microscopy&qsSearchArea=searchText">microscopy; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=titania&qsSearchArea=searchText">titania; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=nanoshells&qsSearchArea=searchText">nanoshells; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=europium%5C%28III%5C%29&qsSearchArea=searchText">europium(III); hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=luminescence&qsSearchArea=searchText">luminescence; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=anatase&qsSearchArea=searchText">anatase; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=HeLa&qsSearchArea=searchText">HeLa; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=polyethyleneimine&qsSearchArea=searchText">polyethyleneimine; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=SEM&qsSearchArea=searchText">SEM; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=TEM&qsSearchArea=searchText">TEM; hors" href="http://pubs.acs.org/action/doSearch?action=search&searchText=DLS&qsSearchArea=searchText">DLS