Hierarchical Polymer Brush Nanoarrays: A Versatile Way to Prepare Multiscale Patterns of Proteins

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• 作者：Yunfeng Li ; Junhu Zhang ; Wendong Liu ; Daowei Li ; Liping Fang ; Hongchen Sun ; Bai Yang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2013
• 出版时间：March 27, 2013
• 年：2013
• 卷：5
• 期：6
• 页码：2126-2132
• 全文大小：403K
• 年卷期：v.5,no.6(March 27, 2013)
• ISSN：1944-8252

文摘

This paper presents a versatile way to prepare multiscale and gradient patterns of proteins. The protein patterns are fabricated by conjugating proteins covalently on patterns of polymer brush that are prepared by techniques combining colloidal lithography with photolithography, and two-step colloidal lithography. Taking advantages of this technique, the parameters of protein patterns, such as height, diameters, periods, and distances between two dots, can be arbitrarily tuned. In addition, the protein patterns with varies of architectures, such as microdiscs, microstripes, microrings, microtriangles, microgrids, etc., consisting of protein nanodots, are prepared and the sample size is up to 4 cm². The as-prepared patterns of fibronectin can promote the cell adhesion and cell location.

Keywords:

protein patterns; colloidal lithography; polymer brush; cell adhesion; multiscale