On-Line Photostability Evaluation System for Chemiluminescence Detection of Phloxine (R-104)

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• 作者：Hiroyuki Nakazawa ; Yumiko Ichikawa ; Takaho Watanabe ; Kayoko Kato ; Yoshihiro Yoshimura ; and Keiko Tanioka-Man
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1997
• 出版时间：December 1997
• 年：1997
• 卷：45
• 期：12
• 页码：4525 - 4529
• 全文大小：246K
• 年卷期：v.45,no.12(December 1997)
• ISSN：1520-5118

文摘

A simple and rapid on-line analytical system was configured for theevaluation of the photostabilityof various food coloring agents constantly exposed to light. Thescreening test for photostability offood coloring agents was carried out in the system consisting of 60 cmof Teflon tubing for ultraviolet(UV) irradiation with a flow rate of mobile phase (water/acetonitrile= 1:1, v/v) at 1 mL/min. Whenthe products derived from photochemical reaction of phloxine (R-104),which is recognized to exhibitchemiluminescence induced by UV irradiation, were separated, severalpeaks were confirmed onthe chromatogram in this system. Furthermore, the peak ofchemilumigenics observed on thechromatogram of photon-counting chemiluminescence detection correspondsto the new peaks onthe UV chromatogram. Also, the presence of phenoxy radical(g = 2.0061) formed by UV irradiationof R-104 was confirmed with an electron spin resonance spectrometer,indicating a reasonablerelationship (r = 0.9041) between the formation of freeradical and the chemiluminescencephenomenon.Keywords: Photostability; phloxine; chemiluminescence; on-line system; ESR;free radical formation