Azoarcus sp. strain DN11 is a denitrifying bacteriumcapable of benzene degradation under anaerobic conditions.The present study evaluated strain DN11 for its applicationto bioaugmentation of benzene-contaminated undergroundaquifers. Strain DN11 could grow on benzene, toluene,
m-xylene, and benzoate as the sole carbon and energysources under nitrate-reducing conditions, although
o- and
p-xylenes were transformed in the presence of toluene.Phenol was not utilized under anaerobic conditions. Kineticanalysis of anaerobic benzene degradation estimated itsapparent affinity and inhibition constants to be 0.82 and 11
M, respectively. Benzene-contaminated groundwatertaken from a former coal-distillation plant site wasanaerobically incubated in laboratory bottles and supplemented with either inorganic nutrients (nitrogen, phosphorus,and nitrate) alone, or the nutrients plus strain DN11,showing that benzene was significantly degraded onlywhen DN11 was introduced. Denaturing gradient gelelectrophoresis of PCR-amplified 16S rRNA gene fragments,and quantitative PCR revealed that DN11 decreasedafter benzene was degraded. Following the decrease inDN11 16S rRNA gene fragments corresponding to bacteriarelated to
Owenweeksia hongkongensis and
Pelotomaculumisophthalicum, appeared as strong bands, suggestingpossible metabolic interactions in anaerobic benzenedegradation. Results suggest that DN11 is potentially usefulfor degrading benzene that contaminates undergroundaquifers at relatively low concentrations.