Development of NIR Fluorescent Dyes Based on Si鈥搑hodamine for in Vivo Imaging
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- 作者：Yuichiro Koide ; Yasuteru Urano ; Kenjiro Hanaoka ; Wen Piao ; Moriaki Kusakabe ; Nae Saito ; Takuya Terai ; Takayoshi Okabe ; Tetsuo Nagano
- 刊名：The Journal of the American Chemical Society
- 出版年：2012
- 出版时间：March 21, 2012
- 年：2012
- 卷：134
- 期：11
- 页码：5029-5031
- 全文大小：225K
- 年卷期：v.134,no.11(March 21, 2012)
- ISSN：1520-5126
文摘
We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si鈥搑hodamine scaffold to obtain emission in the range suitable for in vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency in aqueous media. Both antibody-bound and free dye exhibited high tolerance to photobleaching in aqueous solution. Subcutaneous xenograft tumors were successfully visualized in a mouse tumor model using SiR700-labeled anti-tenascin-C (TN-C) antibody, SiR700-RCB1. SiR-NIRs are expected to be useful as labeling agents for in vivo imaging studies including multicolor imaging, and also as scaffolds for NIR fluorescence probes.