Spatially Controlled DNA Nanopatterns by “Click” Chemistry Using Oligonucleotides with Different Anchoring Sites

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• 作者：Guangyan Qing ; Hai Xiong ; Frank Seela ; Taolei Sun
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：November 3, 2010
• 年：2010
• 卷：132
• 期：43
• 页码：15228-15232
• 全文大小：394K
• 年卷期：v.132,no.43(November 3, 2010)
• ISSN：1520-5126

文摘

DNA patterning on surfaces has broad applications in biotechnology, nanotechnology, and other fields of life science. The common patterns make use of the highly selective base pairing which might not be stable enough for further manipulations. Furthermore, the fabrication of well-defined DNA nanostructures on solid surfaces usually lacks chemical linkages to the surface. Here we report a template-free strategy based on “click” chemistry to fabricate spatially controlled DNA nanopatterns immobilized on surfaces. The self-assembly process utilizes DNA with different anchoring sites. The position of anchoring is of crucial importance for the self-assembly process of DNA and greatly influences the assembly of particular DNA nanopatterns. It is shown that the anchoring site in a central position generates tunable nanonetworks with high regularity, compared to DNAs containing anchoring sites at terminal and other positions. The prepared patterns may find applications in DNA capturing and formation of pores and channels and can serve as templates for the patterning using other molecules.