Controlled Microchannelling in Dense Collagen Scaffolds by Soluble Phosphate Glass Fibers

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• 作者：Showan N. Nazhat ; Ensanya A. Abou Neel ; Asmeret Kidane ; Ifty Ahmed ; Chris Hope ; Matt Kershaw ; Peter D. Lee ; Eleanor Stride ; Nader Saffari ; Jonathan C. Knowles ; Robert A. Brown
• 刊名：Biomacromolecules
• 出版年：2007
• 出版时间：February 2007
• 年：2007
• 卷：8
• 期：2
• 页码：543 - 551
• 全文大小：1064K
• 年卷期：v.8,no.2(February 2007)
• ISSN：1526-4602

文摘

A problem with tissue engineering scaffolds is maintaining seeded cell viability and function due to limitationsof oxygen and nutrient transfer. An approach to maintain suitable oxygen concentrations throughout the scaffoldwould be to controllably incorporate microchannelling within these scaffolds. This study investigated theincorporation of unidirectionally aligned soluble phosphate based glass fibers (PGF) into dense collagen scaffolds.PGF are degradable, and their degradation can be controlled through their chemistry and dimensions. Plasticcompression was used to produce composite scaffolds at three different weight percentage while maintaininggreater than 80% resident cell viability. PGF-collagen scaffold composition was quantified through thermogravimetric analysis as well as being morphologically and mechanically characterized. PGF degradation was measuredthrough ion chromatography, and channel formation was verified with ultrasound imaging and SEM. The freemovement of coated microbubble agents confirmed the channels to be continuous in nature and of 30-40 mdiameter. These microchannels in dense native collagen matrices could play an important role in hypoxia/perfusionlimitations and also in the transportation of nutrients and potentially forming blood vessels through dense implants.