Paramagnetic Relaxation-Based 19F MRI Probe To Detect Protease Activity

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• 作者：Shin Mizukami ; Rika Takikawa ; Fuminori Sugihara ; Yuichiro Hori ; Hidehito Tochio ; Markus Wä ; lchli ; Masahiro Shirakawa ; Kazuya Kikuchi
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：January 23, 2008
• 年：2008
• 卷：130
• 期：3
• 页码：794 - 795
• 全文大小：69K
• 年卷期：v.130,no.3(January 23, 2008)
• ISSN：1520-5126

文摘

A novel design principle for ¹⁹F MRI probes detecting protease activity was developed. This principle is based on ¹⁹F MRI signal quenching by the intramolecular paramagnetic effect from Gd³⁺. The intramolecular Gd³⁺ dramatically attenuated the ¹⁹F probe signal, and the paramagnetic effect was cancelled by the probe hydrolyzation by caspase-3. Using this probe, it was shown that the probe could detect caspase-3 activity spatially from a phantom image using ¹⁹F MRI.