Arsenic Induced Overexpression of Inflammatory Cytokines Based on the Human Urothelial Cell Model in Vitro and Urinary Secretion of Individuals Chronically Exposed to Arsenic

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• 作者：Shengnan Liu ; Qingshan Sun ; Fei Wang ; Lin Zhang ; Yingli Song ; Shuhua Xi ; Guifan Sun
• 刊名：Chemical Research in Toxicology
• 出版年：2014
• 出版时间：November 17, 2014
• 年：2014
• 卷：27
• 期：11
• 页码：1934-1942
• 全文大小：501K
• ISSN：1520-5010

文摘

Chronic persistent inflammation could play an important role in the pathogenesis of some malignancies, and inflammation is a critical factor for bladder cancer development. In this study, we measured urine levels of transforming growth factor-伪 (TGF-伪), tumor necrosis factor-伪 (TNF-伪), and IL-8 in arsenic exposure workers and expressions of inflammatory cytokines in human urothelial cells in vivo and in vitro. We found the concentrations of IL-8, TNF-伪, and TGF-伪 presented in urine were significantly elevated in the high urinary arsenic workers compared with the low urinary arsenic workers. Multiple regression analysis showed that the urinary IL-8 level was significantly positively associated with urinary iAs concentration after adjusting for the confounding effects of age, employed years, body mass index (BMI), smoking, alcohol, and seafood consumption in recent 3 days. Urinary TNF-伪 and TGF-伪 levels were also significantly positively associated with urinary iAs concentration, and SMI. TGF-伪 level was negatively associated with age after adjusting for the confounding effects. Consistent with the results in vivo, mRNA expressions of TNF-伪, TGF-伪, and IL-8 and protein expressions of TGF-伪, TGF-尾1, and IL-8 were significantly elevated in SV-HUC-1 cells after exposure to lower concentrations of arsenite for 24h as compared to the control group. These data indicated that arsenic increased the secretion of inflammatory factors and IL-8, TNF-伪, and TGF-伪 expression may be a useful biomarker of the effect of arsenic exposure.