Implication of the Stereoisomers of Ginsenoside Derivatives in the Antiproliferative Effect of HSC-T6 Cells
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- 作者：Heejung Yang ; Guijae Yoo ; Hye Seong Kim ; Jeom Yong Kim ; Sun Ok Kim ; Young Hyo Yoo ; Sang Hyun Sung
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：November 28, 2012
- 年：2012
- 卷：60
- 期：47
- 页码：11759-11764
- 全文大小：273K
- 年卷期：v.60,no.47(November 28, 2012)
- ISSN：1520-5118
文摘
Two ginsenoside derivatives (9, 10) along with 10 known ginsenosides (1鈥?b>8, 11, and 12) were isolated from BST204, which is a crude ginseng extract fermented by enzyme and acid hydrolysis. The two ginsenosides were determined as 12尾,20(S),25-trihydroxydammara-3-O-尾-d-glucopyranoside (9) and 12尾,20(R),25-trihydroxydammara-3-O-尾-d-glucopyranoside (10). Compounds 1鈥?b>12 were categorized into stereoisomeric pairs differentiated by R- or S-configuration at C-20, the number or position of sugar residues at C-3 or C-6, and the type of derivative at C-21. Their structure鈥揳ctivity relationship was evaluated by the cell viability assay using HSC-T6 cells. Results showed that 20(S) (3 > 4, 7 > 8, and 9 > 10), a 2-hydroxy-2-methylbutyl moiety at C-21 (3, 7 > 9), and the number of sugar residues at C-3 (3 > 7) significantly affected the antiproliferative activity on HSC-T6 cells. The inhibition of the cell proliferation of compound 3 was assessed by annexin-V/PI staining analysis using flow cytometry.