Chromosome 7-Centric Analysis of Proteomics Data from a Panel of Human Colon Carcinoma Cell Lines
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- 作者：Susan Fanayan ; Joshua T. Smith ; Manveen K. Sethi ; David Cantor ; Robert Goode ; Richard J. Simpson ; Mark S. Baker ; William S. Hancock ; Edouard Nice
- 刊名：Journal of Proteome Research
- 出版年：2013
- 出版时间：January 4, 2013
- 年：2013
- 卷：12
- 期：1
- 页码：89-96
- 全文大小：475K
- 年卷期：v.12,no.1(January 4, 2013)
- ISSN：1535-3907
文摘
In this manuscript, we describe a shotgun proteomics approach for a comprehensive proteomic analysis of samples including total lysates, membrane, secretome, and exosome fractions from a panel of colorectal cancer cell lines. We will present an analysis of our proteomics data in two alternative formats. First we will discuss a traditional analysis of our data, in which we identify a number of cancer-associated proteins using various proteomic data analysis tools. In a second approach, we use a chromosome format to organize the proteomic data on chromosome 7, allowing the identification of clusters of cancer-associated genes with boundaries defined by physical proximity on different chromosomes.