Composition of a Chemopreventive Proanthocyanidin-Rich Fraction from Cranberry Fruits Responsible for the Inhibition of 12-O-Tetradecanoyl Phorbol-13-acetate (TPA)-Induced Ornithine Decarboxyla

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• 作者：Fayez E. Kandil ; Mary Ann L. Smith ; Randy B. Rogers ; Marie-France Pé ; pin ; Lynda L. Song ; John M. Pezzuto ; and David S. Seigler
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2002
• 出版时间：February 27, 2002
• 年：2002
• 卷：50
• 期：5
• 页码：1063 - 1069
• 全文大小：86K
• 年卷期：v.50,no.5(February 27, 2002)
• ISSN：1520-5118

文摘

Phenolics from the American cranberry (Vaccinium macrocarpon) were fractionated into a series ofproanthocyanidins and other flavonoid compounds by vacuum chromatography on a hydrophilic,porous polyvinylic gel permeation polymer. Antioxidant activity was not restricted to a particular classof components in the extract but was found in a wide range of the fractions. Significant chemopreventive activity, as indicated by an ornithine decarboxylase assay, was localized in one particularproanthocyanidin-rich fraction from the initial fractionation procedure. Further fractionation of the activeanticarcinogenic fraction revealed the following components: seven flavonoids, mainly quercetin,myricetin, the corresponding 3-O-glycosides, (-)-epicatechin, (+)-catechin, and dimers of bothgallocatechin and epigallocatechin types, and a series of oligomeric proanthocyanidins.Keywords: Anticarcinogenic activity; antioxidants; catechin; condensed tannins; cranberry; epicatechin; FRAP assay; ODC assay; proanthocyanidins; Vaccinium macrocarpon Ait.