Fully Enzymatic Membraneless Glucose|Oxygen Fuel Cell That Provides 0.275 mA cm‿ in 5 mM Glucose, Operates in Human Physiological Solutions, and Powers Transmission of Sensing Data
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- 作者：Peter Ó Conghaile ; Magnus Falk ; Domhnall MacAodha ; Maria E. Yakovleva ; Christoph Gonaus ; Clemens K. Peterbauer ; Lo Gorton ; Sergey Shleev ; Dónal Leech
- 刊名：Analytical Chemistry
- 出版年：2016
- 出版时间：February 16, 2016
- 年：2016
- 卷：88
- 期：4
- 页码：2156-2163
- 全文大小：464K
- ISSN：1520-6882
文摘
Coimmobilization of pyranose dehydrogenase as an enzyme catalyst, osmium redox polymers [Os(4,4′-dimethoxy-2,2′-bipyridine)2(poly(vinylimidazole))10Cl]+ or [Os(4,4′-dimethyl-2,2′-bipyridine)2(poly(vinylimidazole))10Cl]+ as mediators, and carbon nanotube conductive scaffolds in films on graphite electrodes provides enzyme electrodes for glucose oxidation. The recombinant enzyme and a deglycosylated form, both expressed in Pichia pastoris, are investigated and compared as biocatalysts for glucose oxidation using flow injection amperometry and voltammetry. In the presence of 5 mM glucose in phosphate-buffered saline (PBS) (50 mM phosphate buffer solution, pH 7.4, with 150 mM NaCl), higher glucose oxidation current densities, 0.41 mA cm–2, are obtained from enzyme electrodes containing the deglycosylated form of the enzyme. The optimized glucose-oxidizing anode, prepared using deglycosylated enzyme coimmobilized with [Os(4,4′-dimethyl-2,2′-bipyridine)2(poly(vinylimidazole))10Cl]+ and carbon nanotubes, was coupled with an oxygen-reducing bilirubin oxidase on gold nanoparticle dispersed on gold electrode as a biocathode to provide a membraneless fully enzymatic fuel cell. A maximum power density of 275 μW cm–2 is obtained in 5 mM glucose in PBS, the highest to date under these conditions, providing sufficient power to enable wireless transmission of a signal to a data logger. When tested in whole human blood and unstimulated human saliva maximum power densities of 73 and 6 μW cm–2 are obtained for the same fuel cell configuration, respectively.