Paper-Based Device for Rapid Visualization of NADH Based on Dissolution of Gold Nanoparticles

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• 作者：Pingping Liang ; Haixiang Yu ; Bhargav Guntupalli ; Yi Xiao
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：July 15, 2015
• 年：2015
• 卷：7
• 期：27
• 页码：15023-15030
• 全文大小：320K
• ISSN：1944-8252

文摘

We describe a paper-based device that enables rapid and sensitive room-temperature detection of dihydronicotinamide adenine dinucleotide (NADH) via a colorimetric readout and demonstrate its value for monitoring NAD⁺-driven enzymatic reactions. Our system is based on NADH-mediated inhibition of gold nanoparticle (AuNPs) dissolution in a Au³⁺-cetyltrimethylammonium bromide (CTAB) solution. We fabricated a device consisting of a mixed cellulose ester paper featuring a wax-encircled, AuNP-coated film atop a cotton absorbent layer sandwiched between two plastic cover layers. In the absence of NADH, the Au³⁺-CTAB complex dissolves the AuNP layer completely, generating a white color in the test zone. In the presence of NADH, Au³⁺ is rapidly reduced to Au⁺, greatly decreasing the dissolution of AuNPs and yielding a red color that becomes stronger at increasing concentrations of NADH. This device exploits capillary force-assisted vertical diffusion, allowing us to apply a 25 渭L sample to a surface-confined test zone to achieve a detection limit of 12.5 渭M NADH. We used the enzyme glucose dehydrogenase as a model to demonstrate that our paper-based device can monitor NAD⁺-driven biochemical processes with and without selective dehydrogenase inhibitors by naked-eye observation within 4 min at room temperature in a small sample volume. We believe that our paper-based device could offer a valuable and low-cost analytical tool for monitoring NAD⁺-associated enzymatic reactions and screening for dehydrogenase inhibitors in a variety of testing contexts.