文摘
Penicillium digitatum has been cultured on citrus fruits and yeast extract sucrose agar media (YES).Cultivation of fungal cultures on solid medium allowed the isolation of two novel tryptoquivaline-likemetabolites, tryptoquialanine A (1) and tryptoquialanine B (2), also biosynthesized on citrus fruits.Their structural elucidation is described on the basis of their spectroscopic data, including those from2D NMR experiments. The analysis of the biomass sterols led to the identification of 8-12. Fungalinfection on the natural substrates induced the release of citrus monoterpenes together with fungalvolatiles. The host-pathogen interaction in nature and the possible biological role of citrus volatilesare also discussed.Keywords: Citrus fruits; Penicillium digitatum; quivaline metabolites; tryptoquivaline; P. digitatumsterols; phenylacetic acid derivatives; volatile metabolites