Microcystin-LR Promotes Melanoma Cell Invasion and Enhances Matrix Metalloproteinase-2/-9 Expression Mediated by NF-魏B Activation

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• 作者：Xu-Xiang Zhang ; Ziyi Fu ; Zongyao Zhang ; Chen Miao ; Pengfei Xu ; Ting Wang ; Liuyan Yang ; Shupei Cheng
• 刊名：Environmental Science & Technology (ES&T)
• 出版年：2012
• 出版时间：October 16, 2012
• 年：2012
• 卷：46
• 期：20
• 页码：11319-11326
• 全文大小：387K
• 年卷期：v.46,no.20(October 16, 2012)
• ISSN：1520-5851

文摘

This study aimed to explore the molecular mechanisms behind the stimulation effects of microcystin-LR (a well-known cyanobacterial toxin produced in eutrophic lakes or reservoirs) on cancer cell invasion and matrix metalloproteinases (MMPs) expression. Boyden chamber assay showed that microcystin-LR exposure (>12.5 nM) evidently enhanced the invasion ability of the melanoma cells (MDA-MB-435). Tumor Metastasis PCR Array demonstrated that 24 h microcystin-LR treatment (25 nM) caused overexpression of eight genes involved in tumor metastasis, including MMP-2, MMP-9, and MMP-13. Quantitative real-time PCR, Western blotting and gelatin zymography consistently demonstrated that mRNA and protein levels of MMP-2/-9 were increased in the cells after microcystin-LR exposure (P < 0.05 each). Immunofluorescence assay and electrophoretic mobility shift assay revealed that microcystin-LR could activate nuclear factor kappaB (NF-魏B) by accelerating NF-魏B translocation into the nucleus and enhancing NF-魏B binding ability. Furthermore, addition of NF-魏B inhibitor in culture medium could suppress the invasiveness enhancement and MMP-2/-9 overexpression. This study indicates that microcystin-LR can act as a NF-魏B activator to promote MMP-2/-9 expression and melanoma cell invasion, which deserves more environmental health concerns.