Development of the Tracer-Pulse Method for Adsorption Studies of Analyte Mixtures in Liquid Chromatography Utilizing Mass Spectrometric Detection
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- 作者：Jö ; rgen Samuelsson ; Robert Arnell ; Jarle S. Diesen ; Julius Tibbelin ; Alexander Paptchikhine ; Torgny Fornstedt ; Per J. R. Sjö ; berg
- 刊名：Analytical Chemistry
- 出版年：2008
- 出版时间：March 15, 2008
- 年：2008
- 卷：80
- 期：6
- 页码：2105 - 2112
- 全文大小：294K
- 年卷期：v.80,no.6(March 15, 2008)
- ISSN：1520-6882
文摘
The tracer-pulse method provides the real adsorption datapoints directly from simple, straightforward calculationsand is therefore a superior method for multicomponentadsorption isotherm determination in HPLC. Only oneimportant problem has restricted its use so far: the tracerpeaks are invisible using any conventional detectionprinciple. We present a solution to this problem with anapproach with a firm base in analytical chemistry, utilizingstable isotopes and mass spectrometric detection. Thenew approach was used for the determination of binaryadsorption isotherms, and a systematic investigation wasmade of its main sources of error. With this modification,the tracer method can be a prime choice for futurecharacterizations of multicomponent separation systemsand of competitive drug binding studies.