Bioactive Constituents of Cirsium japonicum var. australe
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- 作者：Wan-Chun Lai ; Yang-Chang Wu ; Bal谩zs Dank贸 ; Yuan-Bin Cheng ; Tusty-Jiuan Hsieh ; Chi-Ting Hsieh ; Yu-Chi Tsai ; Mohamed El-Shazly ; Ana Martins ; Judit Hohmann ; Attila Hunyadi ; Fang-Rong Chang
- 刊名：Journal of Natural Products
- 出版年：2014
- 出版时间：July 25, 2014
- 年：2014
- 卷：77
- 期：7
- 页码：1624-1631
- 全文大小：330K
- ISSN：1520-6025
文摘
Cirsium japonicum var. australe, used as a folk medicine in Taiwan, has been employed traditionally in the treatment of diabetes and inflammatory symptoms. Bioactivity-guided fractionation of its ethanolic extract, utilizing centrifugal partition chromatography monitored by DPPH-TLC analysis, led to the isolation of three new acetylenic phenylacrylic acid esters (1鈥?b>3) and two new polyacetylenes (4 and 5), together with seven known compounds (6鈥?b>12). The structures of 1鈥?b>5 were elucidated by spectroscopic methods including 1D and 2D NMR techniques. The absolute configurations of 4 and 7 were determined utilizing Mosher鈥檚 method and ECD/CD experiments. The DPPH scavenging activity of the constituents isolated from the C. japonicum var. australe ethanolic extract was evaluated. The potential antidiabetic activity of some of the isolates was evaluated using in vitro cellular glucose uptake and oil red staining assays.